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从猴垂体中纯化的促卵泡激素制剂的生物学和免疫学特性

Biological and immunological characterization of purified follicle-stimulating hormone preparations from monkey pituitaries.

作者信息

Khan S A, Fröysa B, Diczfalusy E

出版信息

J Med Primatol. 1984;13(5):269-82.

PMID:6439872
Abstract

The suitability of a purified rhesus (rhFSH) and cynomolgus (cynFSH) follicle-stimulating hormone (FSH) preparation to replace the expired LER 1909-2 reference standard in the immunoassay of monkey FSH was assessed using an in vitro bioassay and two heterologous radioimmunoassay (RIA) procedures in combination with fractionation by high-resolution isoelectrofocusing. The results presented indicate that rhFSH is unsuitable for the replacement of LER 1909-2 as a standard, though cynFSH might be suitable after the removal of its LH contamination.

摘要

采用体外生物测定法以及两种异源放射免疫测定(RIA)方法,并结合高分辨率等电聚焦分级分离,评估了纯化的恒河猴(rhFSH)和食蟹猴(cynFSH)促卵泡激素(FSH)制剂在猴FSH免疫测定中替代过期的LER 1909 - 2参考标准品的适用性。给出的结果表明,rhFSH不适于替代LER 1909 - 2作为标准品,不过cynFSH在去除其LH污染后可能适用。

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引用本文的文献

1
In vitro bioassays of follicle-stimulating hormone: methods and clinical applications.促卵泡激素的体外生物测定:方法与临床应用
J Endocrinol Invest. 1991 Dec;14(11):983-97. doi: 10.1007/BF03347131.