Zhou Xiaoling, Yang Jianqing, Liu Ying, Li Zepeng, Yu Jingfang, Wei Wanhua, Chen Qiao, Li Can, Tang Nong
Graduate School of Hunan University of Traditional Chinese Medicine, Changsha, Hunan, China.
Department of Gastroenterology, Liuzhou Traditional Chinese Medicine Hospital, Liuzhou, Guangxi, China.
Braz J Med Biol Res. 2019 Feb 25;52(3):e7879. doi: 10.1590/1414-431X20187879.
Bone marrow mesenchymal stem cells (BMSCs) transplantation has attracted attention for the treatment of liver cirrhosis and end-stage liver diseases. Therefore, in this study, we evaluated the effect of different methods of BMSCs transplantation in the treatment of liver cirrhosis in rats. Seventy-two male Sprague-Dawley rats were divided into 7 groups: 10 were used to extract BMSCs, 10 were used as normal group, and the remaining 52 rats were randomly divided into five groups for testing: control group, BMSCs group, BMSCs+granulocyte colony-stimulating factor (G-CSF) group, and BMSCs+Jisheng Shenqi decoction (JSSQ) group. After the end of the intervention course, liver tissue sections of rats were subjected to hematoxylin and eosin (H&E) and Masson staining, and pathological grades were scored. Liver function [aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB)] and hepatic fibrosis markers [hyaluronidase (HA), laminin (LN), type III procollagen (PCIII), type IV collagen (CIV)] were measured. BMSCs+JSSQ group had the best effect of reducing ALT and increasing ALB after intervention therapy (P<0.05). The reducing pathological scores and LN, PCIII, CIV of BMSCs+G-CSF group and BMSCs+JSSQ group after intervention therapy were significant, but there was no significant difference between the two groups (P>0.05). The effect of JSSQ on improving stem cell transplantation in rats with liver cirrhosis was confirmed. JSSQ combined with BMSCs could significantly improve liver function and liver pathology scores of rats with liver cirrhosis.
骨髓间充质干细胞（BMSCs）移植已在肝硬化和终末期肝病的治疗中受到关注。因此，在本研究中，我们评估了不同方法的BMSCs移植对大鼠肝硬化的治疗效果。将72只雄性Sprague-Dawley大鼠分为7组：10只用于提取BMSCs，10只作为正常组，其余52只大鼠随机分为五组进行试验：对照组、BMSCs组、BMSCs+粒细胞集落刺激因子（G-CSF）组和BMSCs+济生肾气汤（JSSQ）组。干预疗程结束后，对大鼠肝组织切片进行苏木精-伊红（H&E）和Masson染色，并进行病理分级评分。检测肝功能[谷丙转氨酶（ALT）、谷草转氨酶（AST）、白蛋白（ALB）]和肝纤维化标志物[透明质酸酶（HA）、层粘连蛋白（LN）、III型前胶原（PCIII）、IV型胶原（CIV）]。干预治疗后，BMSCs+JSSQ组降低ALT和升高ALB的效果最佳（P<0.05）。干预治疗后，BMSCs+G-CSF组和BMSCs+JSSQ组的病理评分及LN、PCIII、CIV降低显著，但两组间无显著差异（P>0.05）。证实了JSSQ对改善肝硬化大鼠干细胞移植的作用。JSSQ联合BMSCs可显著改善肝硬化大鼠的肝功能和肝脏病理评分。
