Anith K N, Momol M T, Kloepper J W, Marois J J, Olson S M, Jones J B
North Florida Research and Education Center, University of Florida, 155 Research Road, Quincy 32351.
Department of Entomology and Plant Pathology, College of Agriculture, Auburn University, Auburn, AL 36849.
Plant Dis. 2004 Jun;88(6):669-673. doi: 10.1094/PDIS.2004.88.6.669.
Greenhouse experiments were conducted to study the effect of plant growth promoting rhizobacteria (PGPR; Bacillus pumilus SE 34, Pseudomonas putida 89B61, BioYield, and Equity), acibenzolar-S-methyl (Actigard), and a soil amendment with S-H mixture (contains agricultural and industrial wastes such as bagasse, rice husk, oyster shell powder, urea, potassium nitrate, calcium super phosphate, and mineral ash) on bacterial wilt incidence caused by Ralstonia solanacearum (race 1, biovar 1) in susceptible tomato (Lycopersicon esculentum cv. Solar Set). In experiments with PGPR, Pseudomonas putida 89B61 significantly reduced bacterial wilt incidence when applied to the transplants at the time of seeding and 1 week prior to inoculation with Ralstonia solanacearum. BioYield, a formulated PGPR that contained two Bacillus strains, decreased disease significantly in three experiments. Equity, a formulation containing more than 40 different microbial strains, did not reduced wilt incidence compared with the untreated control. With inoculum at low pathogen densities of 1 × 10 and 1 × 10 CFU/ml, disease incidence of Actigard-treated plants was significantly less than with nontreated plants. This is the first report of Actigard-mediated reduction of bacterial wilt incidence in a susceptible tomato cultivar. When PGPR and Actigard applications were combined, Actigard plus P. putida 89B61 or BioYield reduced bacterial wilt incidence compared with the untreated control. Incorporation of S-H mixture into infested soil 2 weeks before transplanting reduced bacterial wilt incidence in one experiment. Combination of Actigard with the S-H mixture significantly reduced bacterial wilt incidence in tomato in two experiments.
进行了温室试验，以研究植物促生根际细菌（PGPR；短小芽孢杆菌SE 34、恶臭假单胞菌89B61、BioYield和Equity）、烯丙苯噻唑（Actigard）以及用S-H混合物（包含甘蔗渣、稻壳、牡蛎壳粉、尿素、硝酸钾、过磷酸钙和矿物灰等农业和工业废料）改良土壤对由青枯雷尔氏菌（1号小种，1型生物变种）引起的易感番茄（番茄品种Solar Set）青枯病发病率的影响。在PGPR试验中，恶臭假单胞菌89B61在播种时施用于移栽苗并在接种青枯雷尔氏菌前1周施用时，显著降低了青枯病发病率。BioYield是一种含有两种芽孢杆菌菌株的复合PGPR，在三项试验中显著降低了病害。Equity是一种含有40多种不同微生物菌株的制剂，与未处理对照相比，并未降低青枯病发病率。当接种病原菌密度为1×10和1×10 CFU/ml的低菌量时，Actigard处理植株的发病率显著低于未处理植株。这是关于Actigard介导降低易感番茄品种青枯病发病率的首次报道。当联合施用PGPR和Actigard时，Actigard加恶臭假单胞菌89B61或BioYield与未处理对照相比降低了青枯病发病率。在移栽前2周将S-H混合物掺入受侵染土壤中，在一项试验中降低了青枯病发病率。在两项试验中，Actigard与S-H混合物联合使用显著降低了番茄青枯病发病率。
