Lecoq Hervé, Desbiez Cécile, Wipf-Scheibel Catherine, Girard Myriam
INRA, Station de Pathologie Végétale, B.P. 94, 84143 Montfavet Cedex, France.
Plant Dis. 2003 Aug;87(8):955-959. doi: 10.1094/PDIS.2003.87.8.955.
Papaya ringspot virus (PRSV) and Zucchini yellow mosaic virus(ZYMV) are potyviruses frequently reported in cucurbits in Mediterranean, subtropical, and tropical regions. Occasionally, epidemics are also observed in more temperate regions, but the ways these viruses are introduced into new areas are not yet fully determined. We investigated the possibility that infected imported melon fruit could be a route for the introduction of PRSV and ZYMV. Imported melon fruits of the yellow canary type infected by ZYMV and PRSV were exposed in the fields next to healthy melon or squash bait plants. During this period, aphids were observed landing and probing on the fruits. In four independent experiments using different fruits, 3.1 to 25% of bait plants were infected by ZYMV and/or PRSV. PRSV was more frequently transmitted to bait plants than ZYMV. Comparison of partial sequences of the isolates from fruits and from bait plants showed a very high, if not complete, identity within each experiment, confirming that a natural transmission did occur from the fruit to the bait plants. These results suggest that globalization of melon production and international trade may be a factor in the spread of cucurbit potyviruses between countries or continents.
番木瓜环斑病毒（PRSV）和小西葫芦黄花叶病毒（ZYMV）是在地中海、亚热带和热带地区葫芦科作物中经常报道的马铃薯Y病毒属病毒。偶尔，在温带地区也会观察到疫情，但这些病毒传入新地区的途径尚未完全确定。我们调查了受感染的进口甜瓜果实可能是PRSV和ZYMV传入途径的可能性。将感染了ZYMV和PRSV的黄色金丝雀型进口甜瓜果实暴露在健康甜瓜或南瓜诱饵植物旁边的田地里。在此期间，观察到蚜虫落在果实上并进行刺探。在使用不同果实的四个独立实验中，3.1%至25%的诱饵植物被ZYMV和/或PRSV感染。PRSV比ZYMV更频繁地传播到诱饵植物上。对果实和诱饵植物分离株的部分序列进行比较，发现在每个实验中，即使不完全相同，也具有非常高的一致性，证实了从果实到诱饵植物确实发生了自然传播。这些结果表明，甜瓜生产的全球化和国际贸易可能是葫芦科马铃薯Y病毒属病毒在国家或大陆之间传播的一个因素。
