Širca S, Urek G, Karssen G
Agricultural Institute of Slovenia, Hacquetova 17, 1001 Ljubjana, Slovenia.
Plant Protection Service, P.O. Box 9102, 6700 HC Wageningen, the Netherlands.
Plant Dis. 2003 Sep;87(9):1150. doi: 10.1094/PDIS.2003.87.9.1150A.
The root-knot nematode Meloidogyne incognita (Kofoid & White) Chitwood is one of the most harmful species within the genus Meloidogyne. It has been detected regularly in greenhouses throughout Europe, while in the field it is restricted to the southern parts of Europe (4). M. incognita is able to parasitize more than 700 plant species and varieties, including the majority of our economically important crops (1). It has been recorded in most parts of the world, including former Yugoslavia; however, there is no data about its presence in Slovenia. In late summer of 2002, large galls were observed on roots of pepper, Capsicum annuum L., grown in a greenhouse located in Portorož near the Adriatic Coast of Slovenia. Different root-knot nematode stages, i.e., males, females, and second-stage juveniles, were isolated and morphologically identified as M. incognita (2). Additional isozyme gel electrophoresis confirmed this (3). To our knowledge, this is the first report of M. incognita from Slovenia. In addition, M. hapla Chitwood was found for the first time in a field in Slovenia. Previously, it had been detected on different host plants, all restricted to greenhouses. In October 2002, M. hapla was isolated and identified from relatively small galls of sweet pepper, C. annuum L., grown in a field near Ljubljana, located in central Slovenia. Additional resampling of the soil comfirmed the presence of M. hapla in this field. References: (1) J. D. Eisenback. Root-Knot Nematode Taxonomic Database. CD-ROM, CABI, Wallingford, UK, 1998. (2) J. D. Eisenback and H. Hirschmann Triantaphyllou. Root-knot nematodes: Meloidogyne species and races. Pages 191-274 in: Manual of Agricultural Nematology. W. R. Nickle, ed. Marcel Dekkers, Inc., New York, 1991. (3) P. R. Esbenshade and A. C. Triantaphyllou. J. Nematol. 17:6, 1985. (4) G. Karssen. Revision of the European root-knot nematodes III on mono- and dicotyledons. Pages 119-121 in: The Plant-Parasitic Nematode Genus Meloidogyne Göldi, 1892 (Tylenchida) in Europe. Koninklijke Brill NV, Leiden, the Netherlands, 2002.
南方根结线虫（Meloidogyne incognita (Kofoid & White) Chitwood）是根结线虫属中危害最大的物种之一。在欧洲各地的温室中经常能检测到它，而在田间它仅局限于欧洲南部地区（4）。南方根结线虫能够寄生于700多种植物物种和品种，包括大多数具有重要经济价值的作物（1）。在世界大部分地区都有其记录，包括前南斯拉夫；然而，斯洛文尼亚没有关于其存在的数据。2002年夏末，在斯洛文尼亚亚得里亚海沿岸附近的科佩尔一个温室种植的辣椒（Capsicum annuum L.）根部观察到了大的虫瘿。分离出了不同的根结线虫阶段，即雄虫、雌虫和二龄幼虫，并通过形态学鉴定为南方根结线虫（2）。额外的同工酶凝胶电泳证实了这一点（3）。据我们所知，这是斯洛文尼亚首次报道南方根结线虫。此外，北方根结线虫（Meloidogyne hapla Chitwood）首次在斯洛文尼亚的一块田地里被发现。此前，它已在不同寄主植物上被检测到，所有这些寄主植物都局限于温室。2002年10月，从斯洛文尼亚中部卢布尔雅那附近一块田地里种植的甜椒（C. annuum L.）相对较小的虫瘿中分离并鉴定出北方根结线虫。对土壤的再次采样证实了该田地中存在北方根结线虫。参考文献：（1）J. D. Eisenback。根结线虫分类数据库。光盘，国际应用生物科学中心，英国沃灵福德，1998年。（2）J. D. Eisenback和H. Hirschmann Triantaphyllou。根结线虫：南方根结线虫的物种和生理小种。载于：《农业线虫学手册》，W. R. Nickle编。马塞尔·德克尔公司，纽约，1991年，第191 - 274页。（3）P. R. Esbenshade和A. C. Triantaphyllou。《线虫学杂志》17:6，1985年。（4）G. Karssen。欧洲根结线虫III在单子叶植物和双子叶植物上的修订。载于：《植物寄生线虫属南方根结线虫Göldi，1892（垫刃目）在欧洲》。荷兰莱顿皇家布里尔出版社，2002年，第119 - 121页。
