Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília 70910-900, Brazil.
Laboratory of Virology, Wageningen University (WUR), Wageningen 6708 PB, Netherlands.
Viruses. 2019 Feb 22;11(2):187. doi: 10.3390/v11020187.
Tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV) share several genetic and biological traits. Both of them belong to the genus (family ), which is composed by viruses with tripartite RNA genome that infect plants and are transmitted by thrips (order Thysanoptera). Previous studies have suggested several reassortment events between these two viruses, and some speculated that they may share one of their genomic segments. To better understand the intimate evolutionary history of these two viruses, we sequenced the genomes of the first TCSV and GRSV isolates ever reported. Our analyses show that TCSV and GRSV isolates indeed share one of their genomic segments, suggesting that one of those viruses may have emerged upon a reassortment event. Based on a series of phylogenetic and nucleotide diversity analyses, we conclude that the parental genotype of the M segment of TCSV was either eliminated due to a reassortment with GRSV or it still remains to be identified.
番茄斑萎病毒（TCSV）和花生环斑病毒（GRSV）具有几个遗传和生物学特征。它们都属于 （科）属，该属由具有三分体 RNA 基因组的病毒组成，感染植物并通过蓟马（缨翅目）传播。先前的研究表明这两种病毒之间发生了几次重配事件，一些人推测它们可能共享基因组的一个片段。为了更好地了解这两种病毒的密切进化历史，我们首次对 TCSV 和 GRSV 分离株的基因组进行了测序。我们的分析表明，TCSV 和 GRSV 分离株确实共享其基因组的一个片段，表明其中一种病毒可能是通过重配事件产生的。基于一系列系统发育和核苷酸多样性分析，我们得出结论，TCSV 的 M 片段的亲代基因型要么由于与 GRSV 的重配而被消除，要么仍有待确定。
