United States Department of Agriculture-Agricultural Research Service, U.S. Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL 34945, USA.
Virology. 2011 May 10;413(2):216-25. doi: 10.1016/j.virol.2011.02.011. Epub 2011 Mar 6.
Reassortment allows multicomponent viruses to exchange genome segments, a process well-documented in the vertebrate- and arthropod-infecting members of the family Bunyaviridae but not between distinct species of the plant- and insect-infecting members of the genus Tospovirus. Genome sequence comparisons of a virus causing severe tospovirus-like symptoms in Florida tomato with Groundnut ringspot virus (GRSV) and Tomato chlorotic spot virus (TCSV) demonstrated that reassortment has occurred, with the large (L) and small (S) RNAs coming from GRSV and the medium (M) RNA coming from TCSV (i.e. L(G)M(T)S(G)). Neither parental genotype is known to occur in the U.S. suggesting that L(G)M(T)S(G) was introduced as a reassortant. L(G)M(T)S(G) was transmitted by western flower thrips (Frankliniella occidentalis [Pergande]), and was not able to overcome the Sw5 resistance gene of tomato. Our demonstration of reassortment between GRSV and TCSV suggests caution in defining species within the family Bunyaviridae based on their ability to reassort.
重配使多组分病毒能够交换基因组片段，这一过程在脊椎动物和节肢动物感染的布尼亚病毒科成员中得到了很好的记录，但在植物和昆虫感染的托斯病毒属成员之间并没有发生。对佛罗里达州番茄中引起严重托斯病毒样症状的病毒与花生环斑病毒（GRSV）和番茄黄化斑点病毒（TCSV）的基因组序列比较表明，已经发生了重配，大（L）和小（S）RNA 来自 GRSV，中（M）RNA 来自 TCSV（即 L(G)M(T)S(G)）。在美国既不知道亲本体基因型的存在，也没有发生 L(G)M(T)S(G)，这表明 L(G)M(T)S(G)是作为重组体引入的。L(G)M(T)S(G)由西部花蓟马（Frankliniella occidentalis [Pergande]）传播，并且不能克服番茄的 Sw5 抗性基因。我们证明了 GRSV 和 TCSV 之间的重配，这表明在基于其重配能力定义布尼亚病毒科内的物种时应谨慎。
