Xu G L, Sivarajah K, Wu R, Nettesheim P, Eling T
Exp Lung Res. 1986;10(1):101-14. doi: 10.3109/01902148609057506.
The metabolism of arachidonic acid to prostaglandins and thromboxane by freshly isolated and cultured respiratory tract epithelial cells was examined by HPLC methods. Homogenates prepared from freshly isolated rat and rabbit tracheal epithelial cells did not convert arachidonic acid to prostaglandins. Rat tracheal epithelial cells however, did convert arachidonic acid to uncharacterized metabolites possibly hydroxyfatty acids. In contrast, rat tracheal epithelial cells grown in culture for 9 days acquired the capacity to convert arachidonic acid to PGE2 and related products while cultured rabbit tracheal epithelial cells converted arachidonic acid to TXB2. The conversion of arachidonic acid to PGE2 by rat tracheal cells was studied at various times in culture. The formation of PGE2 appeared to parallel the growth of the cultures. In contrast to freshly isolated rat tracheal cells, enriched rat Clara cell fractions were able to convert 14C-arachidonic acid to prostacyclin (PGI2) ans measured by HPLC analysis of its stable end product 6-keto PGF1 alpha. PGI2 was also the major metabolite of arachidonic acid produced by enriched rat alveolar type II cell fractions. PGF2 alpha, and hydroxyfatty acids were also formed. These results suggest that arachidonic acid metabolism differs in various types of respiratory tract cells and that maintenance of such cells in culture alters the pattern of arachidonic acid metabolism.
采用高效液相色谱法检测新鲜分离及培养的呼吸道上皮细胞中花生四烯酸向前列腺素和血栓素的代谢情况。由新鲜分离的大鼠和兔气管上皮细胞制备的匀浆不能将花生四烯酸转化为前列腺素。然而，大鼠气管上皮细胞确实能将花生四烯酸转化为未鉴定的代谢产物，可能是羟基脂肪酸。相比之下，培养9天的大鼠气管上皮细胞获得了将花生四烯酸转化为前列腺素E2及相关产物的能力，而培养的兔气管上皮细胞则将花生四烯酸转化为血栓素B2。在培养的不同时间研究了大鼠气管细胞将花生四烯酸转化为前列腺素E2的情况。前列腺素E2的形成似乎与培养物的生长平行。与新鲜分离的大鼠气管细胞不同，通过对其稳定终产物6-酮前列腺素F1α进行高效液相色谱分析测定，富集的大鼠克拉拉细胞组分能够将14C-花生四烯酸转化为前列环素（PGI2）。PGI2也是富集的大鼠肺泡Ⅱ型细胞组分产生的花生四烯酸的主要代谢产物。还形成了前列腺素F2α和羟基脂肪酸。这些结果表明，花生四烯酸代谢在呼吸道的不同类型细胞中存在差异，并且在培养中维持此类细胞会改变花生四烯酸的代谢模式。
