Pietilä P, Moilanen E, Seppälä E, Nissilä M, Lepistö P, Laitinen O, Vapaatalo H
Scand J Rheumatol. 1984;13(3):243-6. doi: 10.3109/03009748409100393.
Production of various arachidonic acid metabolites from both endogenous and exogenous substrate was measured using cultures of synovial fibroblasts from healthy and rheumatic synovia. At first, the rheumatic cells showed retarded growth and an altered histological picture. Rheumatic cells produced more 6-keto-PGF1 alpha, the main metabolite of prostacyclin, and prostaglandin E2 than did normal cells, which synthesized more thromboxane B2. Later on these differences diminished or disappeared, except regarding 6-keto-PGF1 alpha. When fairly high concentrations of exogenous arachidonic acid were used, for 2-hour incubation of the cells, the production of identified metabolites, 6-keto-PGF1 alpha, PGF2 alpha, PGE2, PGD2, PGA + PGB and thromboxane B2, was slightly less in rheumatic cells. In general, the main metabolite formed was 6-keto-PGF1 alpha. Some kind of feedback mechanism between prostaglandins and cyclic nucleotides is suggested.
利用来自健康和风湿性滑膜的滑膜成纤维细胞培养物，测定了内源性和外源性底物产生的各种花生四烯酸代谢物。起初，风湿性细胞生长迟缓，组织学图像改变。风湿性细胞产生的前列环素主要代谢物6-酮-前列环素F1α和前列腺素E2比正常细胞多，而正常细胞合成的血栓素B2更多。后来，除了6-酮-前列环素F1α外，这些差异减小或消失。当使用相当高浓度的外源性花生四烯酸对细胞进行2小时孵育时，风湿性细胞中已鉴定的代谢物6-酮-前列环素F1α、前列腺素F2α、前列腺素E2、前列腺素D2、前列腺素A + 前列腺素B和血栓素B2的产生量略少。一般来说，形成的主要代谢物是6-酮-前列环素F1α。提示前列腺素和环核苷酸之间存在某种反馈机制。
