Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.
Veterans Affairs Tennessee Valley Healthcare System, Nashville, TN, USA.
Sci Rep. 2019 Feb 27;9(1):2882. doi: 10.1038/s41598-019-39232-z.
Crohn's disease (CD) has been associated with an increased consumption of n-6 polyunsaturated fatty acid (PUFA), while greater intake of n-3 PUFA has been associated with a reduced risk. We sought to investigate serum fatty acid composition in CD, and associations of fatty acids with disease activity, cytokines, and adipokines. Serum was prospectively collected from 116 CD subjects and 27 non-IBD controls. Clinical disease activity was assessed by the Harvey Bradshaw Index (HBI). Serum fatty acids were measured by gas chromatography. Serum cytokines and adipokines were measured by Luminex assay. Dietary histories were obtained from a subset of patients. Nine serum cytokines and adipokines were increased in CD versus controls. CD subjects had increased percentage serum monounsaturated fatty acids (MUFA), dihomo-gamma linolenic acid (DGLA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and oleic acid, but decreased arachidonic acid (AA) versus controls. The % total n-3 fatty acids and % EPA directly correlated with pro-inflammatory cytokine levels and HBI, whereas the % total n-6 fatty acids were inversely correlated with pro-inflammatory cytokine levels and HBI. CD subjects had increased caloric intake versus controls, but no alterations in total fat or PUFA intake. We found differences in serum fatty acids, most notably PUFA, in CD that correlated both with clinical disease activity and inflammatory cytokines. Our findings indicate that altered fatty acid metabolism or utilization is present in CD and is related to disease activity.
克罗恩病（CD）与 n-6 多不饱和脂肪酸（PUFA）的消耗增加有关，而 n-3 PUFA 的摄入增加与风险降低有关。我们旨在研究 CD 患者的血清脂肪酸组成，并探讨脂肪酸与疾病活动、细胞因子和脂肪因子的相关性。从 116 例 CD 患者和 27 例非 IBD 对照者中前瞻性收集血清。采用 Harvey Bradshaw 指数（HBI）评估临床疾病活动度。通过气相色谱法测定血清脂肪酸。采用 Luminex 检测试剂盒测定血清细胞因子和脂肪因子。从部分患者中获得饮食史。与对照组相比，CD 患者有 9 种血清细胞因子和脂肪因子升高。与对照组相比，CD 患者血清单不饱和脂肪酸（MUFA）、二高-γ-亚麻酸（DGLA）、二十碳五烯酸（EPA）、二十二碳五烯酸（DPA）和油酸的百分比增加，而花生四烯酸（AA）的百分比降低。总 n-3 脂肪酸和 EPA 的百分比与促炎细胞因子水平和 HBI 直接相关，而总 n-6 脂肪酸的百分比与促炎细胞因子水平和 HBI 呈负相关。与对照组相比，CD 患者的热量摄入增加，但总脂肪或 PUFA 摄入没有变化。我们发现 CD 患者的血清脂肪酸存在差异，尤其是 PUFA，与临床疾病活动和炎症细胞因子密切相关。我们的研究结果表明，CD 患者存在脂肪酸代谢或利用的改变，且与疾病活动相关。
