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用于摄取研究的细胞外囊泡亲脂性荧光团染色相关的陷阱。

Pitfalls associated with lipophilic fluorophore staining of extracellular vesicles for uptake studies.

作者信息

Simonsen Jens B

机构信息

Department of Health Technology (DTU Health Tech) and Department of Micro- and Nanotechnology (DTU Nanotech), Technical University of Denmark, Lyngby, Denmark.

出版信息

J Extracell Vesicles. 2019 Feb 20;8(1):1582237. doi: 10.1080/20013078.2019.1582237. eCollection 2019.

DOI:10.1080/20013078.2019.1582237
PMID:30815239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6383605/
Abstract

Post-staining of extracellular vesicles (EVs) with lipid-anchored fluorophores (LAFs) such as PKH67 is a widely used strategy for studying EVs but it is associated with several pitfalls. The pitfalls discussed in this commentary are related to LAF labelling of non-EV species due to (1) lipoprotein contamination in EV samples, (2) desorption of the LAF reporters from vesicles into proteins and lipoproteins in blood and serum, and (3) the capability of the amphiphilic LAF compounds to form EV-like particles. Awareness of these challenges and developing solutions to overcome these are important to ensure that we make relevant interpretations when using LAFs to track EVs.

摘要

使用脂质锚定荧光团（LAF）（如PKH67）对细胞外囊泡（EV）进行染色后处理是研究EV的一种广泛使用的策略，但它存在几个缺陷。本评论中讨论的缺陷与非EV物种的LAF标记有关，原因如下：（1）EV样本中的脂蛋白污染；（2）LAF报告分子从囊泡解吸到血液和血清中的蛋白质和脂蛋白中；（3）两亲性LAF化合物形成类似EV颗粒的能力。认识到这些挑战并开发克服这些挑战的解决方案，对于确保我们在使用LAF追踪EV时做出相关解释非常重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fbf/6383605/9e56b97dfcd0/ZJEV_A_1582237_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fbf/6383605/9e56b97dfcd0/ZJEV_A_1582237_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fbf/6383605/9e56b97dfcd0/ZJEV_A_1582237_F0001_OC.jpg

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