Epstein R, Sham G, Womack J, Yagüe J, Palmer E, Cohn M
J Exp Med. 1986 Apr 1;163(4):759-73. doi: 10.1084/jem.163.4.759.
The genetic control of the cytotoxic T-cell response to the male histocompatibility antigen, H-Y, was analyzed in BALB/cKe(C) and SJL/J(J) which are both nonresponders. However, the (C X J)F1 hybrid is a responder. Therefore, two dominant complementing genes are involved. Analysis of a set of (C X J) recombinant inbred (RI) lines reveals that these two complementing gene products are a restricting element (R) encoded by the H-2 (MHC) locus on chromosome 17 and a subunit of the T-cell receptor (anti-R) encoded by the Tar alpha-locus on chromosome 14. The order and orientation of gene segments within the Tar alpha-locus has also been established relative to the chromosome 14 marker, Es-10. The existence of two RI strains which are recombinant at chromosome 14 has made it possible to determine that this order is Es-10--v alpha-1--v alpha-2--[C alpha--Np-2]--centromere. The implications of these data for the antigen-specific regulation of immune responsiveness are discussed in terms of the dual recognitive-single receptor model.
在均为无反应者的BALB/cKe(C)和SJL/J(J)小鼠中,分析了细胞毒性T细胞对雄性组织相容性抗原H-Y的反应的遗传控制。然而,(C×J)F1杂种是有反应者。因此,涉及两个显性互补基因。对一组(C×J)重组近交(RI)系的分析表明,这两个互补基因产物是由17号染色体上的H-2(主要组织相容性复合体)基因座编码的一个限制元件(R)和由14号染色体上的Tarα基因座编码的T细胞受体的一个亚基(抗-R)。相对于14号染色体标记Es-10,也确定了Tarα基因座内基因片段的顺序和方向。利用在14号染色体上发生重组的两个RI品系,已确定该顺序为Es-10--vα-1--vα-2--[Cα--Np-2]--着丝粒。根据双识别-单受体模型,讨论了这些数据对免疫反应性抗原特异性调节的意义。