人类T细胞受体Vβ6.7基因产物中的等位基因变异。
Allelic variations in the human T cell receptor V beta 6.7 gene products.
作者信息
Li Y, Szabo P, Robinson M A, Dong B, Posnett D N
机构信息
Department of Medicine, Cornell University Medical College, New York, New York 10021.
出版信息
J Exp Med. 1990 Jan 1;171(1):221-30. doi: 10.1084/jem.171.1.221.
Abstract
Polymorphisms of human TCR gene products have been suggested by the description of a mAb, OT145, that identifies a subset of TCRs in some individuals but not in others (6). Here we demonstrate that this mAb detects a TCR allotype of the V beta 6.7 gene. Two allelic products of this V gene differ by two nonconservative amino acid substitutions. The mAb OT145 appears to react with V beta 6.7 a gene products ("+" allele), but not with V beta 6.7b gene products ("-" allele). This represents the first direct demonstration that TCR V gene allotypes exist and provides a possible explanation for immune responses under the control of TCR V genes and for disease associations with TCR V genes.
摘要
一种单克隆抗体OT145的描述提示了人类TCR基因产物的多态性，该抗体可识别部分个体而非所有个体中的TCR亚群(6)。在此，我们证明该单克隆抗体可检测Vβ6.7基因的一种TCR同种异型。该V基因的两种等位基因产物因两个非保守氨基酸替换而有所不同。单克隆抗体OT145似乎与Vβ6.7a基因产物（“+”等位基因）发生反应，但不与Vβ6.7b基因产物（“-”等位基因）发生反应。这是首次直接证明TCR V基因同种异型的存在，并为TCR V基因控制下的免疫反应以及TCR V基因与疾病的关联提供了一种可能的解释。
相似文献
1
Allelic variations in the human T cell receptor V beta 6.7 gene products.人类T细胞受体Vβ6.7基因产物中的等位基因变异。
J Exp Med. 1990 Jan 1;171(1):221-30. doi: 10.1084/jem.171.1.221.
2
Allelic sequence variations in the hypervariable region of a T-cell receptor beta chain: correlation with restriction fragment length polymorphism in human families and populations.
Proc Natl Acad Sci U S A. 1989 Dec;86(23):9422-6. doi: 10.1073/pnas.86.23.9422.
3
4
Restricted T-cell receptor V beta gene usage by myelin basic protein-specific T-cell clones in multiple sclerosis: predominant genes vary in individuals.多发性硬化症中髓鞘碱性蛋白特异性T细胞克隆对T细胞受体Vβ基因的使用受限：优势基因在个体间存在差异。
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2466-70. doi: 10.1073/pnas.88.6.2466.
5
Systematic study of human alpha beta T cell receptor V segments shows allelic variations resulting in a large number of distinct T cell receptor haplotypes.对人类αβ T细胞受体V基因片段的系统研究显示，等位基因变异导致大量不同的T细胞受体单倍型。
Eur J Immunol. 1993 Jun;23(6):1277-83. doi: 10.1002/eji.1830230613.
6
Flexibility of the T cell repertoire. Self tolerance causes a shift of T cell receptor gene usage in response to insulin.T细胞受体库的灵活性。自身耐受性导致T细胞受体基因使用情况因胰岛素而发生转变。
J Exp Med. 1990 May 1;171(5):1665-81. doi: 10.1084/jem.171.5.1665.
7
Major histocompatibility complex determinants select T-cell receptor alpha chain variable region dominance in a peptide-specific response.主要组织相容性复合体决定因素在肽特异性应答中选择T细胞受体α链可变区优势。
Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):8874-8. doi: 10.1073/pnas.89.19.8874.
8
T cell receptor V beta gene usage in a human alloreactive response. Shared structural features among HLA-B27-specific T cell clones.人同种异体反应中T细胞受体Vβ基因的使用。HLA - B27特异性T细胞克隆之间的共同结构特征。
J Exp Med. 1990 Apr 1;171(4):1189-204. doi: 10.1084/jem.171.4.1189.
9
Genetic differences in the T cell receptor alleles of LEW rats and their encephalomyelitis-resistant derivative, LER, and their impact on the inheritance of EAE resistance.LEW大鼠及其抗脑脊髓炎衍生物LER的T细胞受体等位基因的遗传差异及其对实验性自身免疫性脑脊髓炎抗性遗传的影响。
Eur J Immunol. 1991 Sep;21(9):2033-41. doi: 10.1002/eji.1830210910.
10
Junctional sequences of fetal T cell receptor beta chains have few N regions.胎儿T细胞受体β链的连接序列几乎没有N区。
J Exp Med. 1991 Jul 1;174(1):115-24. doi: 10.1084/jem.174.1.115.
引用本文的文献
1
Diagnostic role of tests for T cell receptor (TCR) genes.T细胞受体（TCR）基因检测的诊断作用
J Clin Pathol. 2003 Jan;56(1):1-11. doi: 10.1136/jcp.56.1.1.
2
Association study of proposed candidate genes/regions in a population of Spanish asthmatics.西班牙哮喘患者群体中拟议的候选基因/区域的关联研究。
Eur J Epidemiol. 2000;16(8):745-50. doi: 10.1023/a:1026758319621.
3
T cell receptor repertoire and function in patients with DiGeorge syndrome and velocardiofacial syndrome.DiGeorge综合征和腭心面综合征患者的T细胞受体库及功能
Clin Exp Immunol. 2000 Jul;121(1):127-32. doi: 10.1046/j.1365-2249.2000.01247.x.
4
TCR binding differs for a bacterial superantigen (SEE) and a viral superantigen (Mtv-9).TCR与一种细菌超抗原（SEE）和一种病毒超抗原（Mtv-9）的结合情况有所不同。
J Exp Med. 1996 Oct 1;184(4):1471-82. doi: 10.1084/jem.184.4.1471.
5
Human T-cell receptor variable gene segment families.人类T细胞受体可变基因片段家族
Immunogenetics. 1995;42(6):455-500. doi: 10.1007/BF00172176.
6
7
Polymorphism and phylogeny of dinucleotide repeats in human T-cell receptor Vb6 genes.人类T细胞受体Vb6基因中二核苷酸重复序列的多态性与系统发育
Immunogenetics. 1993;38(2):92-7. doi: 10.1007/BF00190896.
8
Human T-cell receptor TCRAV, TCRBV, and TCRAJ sequences newly found in T-cell clones reactive with allogeneic HLA class II antigens.在与同种异体 HLA Ⅱ类抗原反应的 T 细胞克隆中新发现的人类 T 细胞受体 TCRAV、TCRBV 和 TCRAJ 序列。
Immunogenetics. 1993;38(1):67-70. doi: 10.1007/BF00216395.
9
Novel members and germline polymorphisms in the human T-cell receptor Vb6 family.人类T细胞受体Vb6家族中的新成员和种系多态性
Immunogenetics. 1993;37(4):257-65. doi: 10.1007/BF00187451.
10
Human T cells respond to mouse mammary tumor virus-encoded superantigen: V beta restriction and conserved evolutionary features.人类T细胞对小鼠乳腺肿瘤病毒编码的超抗原产生反应：Vβ限制和保守的进化特征。
J Exp Med. 1993 Jun 1;177(6):1735-43. doi: 10.1084/jem.177.6.1735.
本文引用的文献
1
Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes.人类胎儿Gγ-和Aγ-珠蛋白基因：完整的核苷酸序列表明，这些重复基因之间可发生DNA交换。
Cell. 1980 Oct;21(3):627-38. doi: 10.1016/0092-8674(80)90426-2.
2
The sequence (dC-dA)n X (dG-dT)n forms left-handed Z-DNA in negatively supercoiled plasmids.序列（dC-dA）n×（dG-dT）n在负超螺旋质粒中形成左手Z-DNA。
Proc Natl Acad Sci U S A. 1983 Apr;80(7):1821-5. doi: 10.1073/pnas.80.7.1821.
3
Negatively supercoiled simian virus 40 DNA contains Z-DNA segments within transcriptional enhancer sequences.负超螺旋猿猴病毒40 DNA在转录增强子序列中包含Z-DNA片段。
Nature. 1983;303(5919):674-9. doi: 10.1038/303674a0.
4
A rapid boiling method for the preparation of bacterial plasmids.一种制备细菌质粒的快速煮沸法。
Anal Biochem. 1981 Jun;114(1):193-7. doi: 10.1016/0003-2697(81)90473-5.
5
A simple and very efficient method for generating cDNA libraries.一种简单且非常有效的生成cDNA文库的方法。
Gene. 1983 Nov;25(2-3):263-9. doi: 10.1016/0378-1119(83)90230-5.
6
Enhanced gene expression by the poly(dT-dG).poly(dC-dA) sequence.由聚（dT-dG）.聚（dC-dA）序列增强的基因表达。
Mol Cell Biol. 1984 Dec;4(12):2622-30. doi: 10.1128/mcb.4.12.2622-2630.1984.
7
Characterization of genomic poly(dT-dG).poly(dC-dA) sequences: structure, organization, and conformation.基因组聚（dT-dG）·聚（dC-dA）序列的表征：结构、组织与构象
Mol Cell Biol. 1984 Dec;4(12):2610-21. doi: 10.1128/mcb.4.12.2610-2621.1984.
8
Structure and diversity of the human T-cell receptor beta-chain variable region genes.人类T细胞受体β链可变区基因的结构与多样性
Science. 1986 Aug 22;233(4766):879-83. doi: 10.1126/science.3755549.
9
T cell tolerance by clonal elimination in the thymus.胸腺中通过克隆清除实现的T细胞耐受性。
Cell. 1987 Apr 24;49(2):273-80. doi: 10.1016/0092-8674(87)90568-x.
10
Secondary, tertiary, and quaternary structure of T-cell-specific immunoglobulin-like polypeptide chains.T细胞特异性免疫球蛋白样多肽链的二级、三级和四级结构。
Proc Natl Acad Sci U S A. 1986 Feb;83(3):742-6. doi: 10.1073/pnas.83.3.742.
Zotero 插件