State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science and Veterinary, Zhejiang Academy of Agricultural Science, Hangzhou, China.
Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Anim Biotechnol. 2024 Nov;35(1):2390940. doi: 10.1080/10495398.2024.2390940. Epub 2024 Aug 13.
Blood composition is indicative of health-related traits such as immunity and metabolism. The use of molecular genetics to investigate alterations in these attributes in laying ducks is a novel approach. Our objective was to employ genome - wide association studies (GWAS) and haplotype - sharing analysis to identify genomic regions and potential genes associated with 11 blood components in Shaoxing ducks. Our findings revealed 35 SNPs and 1 SNP associated with low-density lipoprotein cholesterol (LDL) and globulin (GLB), respectively. We identified 36 putative candidate genes for the LDL trait in close proximity to major QTLs and key loci. Based on their biochemical and physiological properties, , , , , and are the strongest putative candidate genes. Through linkage disequilibrium analysis and haplotype sharing analysis, we identified three haplotypes and one haplotype, respectively, that were significantly linked with LDL and GLB. These haplotypes could be selected as potential candidate haplotypes for molecular breeding of Shaoxing ducks. Additionally, we utilized a bootstrap test to verify the reliability of GWAS with small experimental samples. The test can be accessed at https://github.com/xuwenwu24/Bootstrap-test.
血液成分是反映健康相关特征的指标，如免疫力和新陈代谢。利用分子遗传学研究产蛋鸭这些特性的变化是一种新方法。我们的目的是利用全基因组关联研究（GWAS）和单倍型共享分析来鉴定与绍兴鸭 11 种血液成分相关的基因组区域和潜在基因。研究结果发现了 35 个与低密度脂蛋白胆固醇（LDL）和球蛋白（GLB）相关的 SNP，分别有 1 个。我们在接近主要 QTL 和关键基因座的地方鉴定出 36 个 LDL 性状的候选基因。根据它们的生化和生理特性， 、 、 、 、 和 是最有潜力的候选基因。通过连锁不平衡分析和单倍型共享分析，我们分别鉴定出与 LDL 和 GLB 显著相关的三个单倍型和一个单倍型。这些单倍型可以作为绍兴鸭分子育种的潜在候选单倍型进行选择。此外，我们还利用 bootstrap 测试来验证小实验样本的 GWAS 的可靠性。该测试可在 https://github.com/xuwenwu24/Bootstrap-test 上获取。
