Kita T, Chihara K, Abe H, Minamitani N, Kaji H, Kodama H, Kashio Y, Okimura Y, Fujita T, Ling N
Endocrinology. 1985 Jan;116(1):259-62. doi: 10.1210/endo-116-1-259.
A heterologous RIA for rat GH-releasing factor (rGRF) was established using synthetic rGRF-(1-43)OH for both standard reference and radioiodination with the antiserum produced against human GRF-(1-44) NH2. The regional distribution of rGRF-like immunoreactivity (LI) in rat hypothalamus was examined according to the Palkovits microdissection method and compared with that of somatostatin (SRIF)-LI. Both rGRF- and SRIF-LI contents (mean +/- SE; nanograms per mg protein) were highest in the median eminence (rGRF, 32.28 +/- 11.42; SRIF, 109.9 +/- 19.2) and next most abundant in the arcuate nucleus (rGRF, 3.50 +/- 0.47; SRIF, 12.88 +/- 0.60). Only a small amount of rGRF-LI was found in the ventromedial (1.41 +/- 0.51) and dorsomedial (1.16 +/- 0.15) nuclei and the anterior hypothalamic area (1.29 +/- 0.42), whereas rGRF-LI was not detected in the other nuclei of the hypothalamus. A considerable amount of SRIF-LI was contained in the periventricular, ventromedial, and paraventricular nuclei and the anterior hypothalamic area, in accordance with other reports. Gel filtration chromatography revealed that hypothalamic extracts contained a major peak of rGRF-LI corresponding to rGRF-(1-43)OH and two peaks of SRIF-LI equivalent to SRIF-(1-28) and SRIF-(1-14), respectively. These findings indicate that rGRF-LI is localized in the median eminence and arcuate nucleus in the rat and that rGRF-, SRIF-(1-28)-, and SRIF-(1-14)-LI are present in a 1:2.10:6.29 ratio on a molar basis.
利用合成的大鼠生长激素释放因子（rGRF）-(1 - 43)OH作为标准参照物和用于放射性碘化，并使用针对人GRF-(1 - 44)NH₂产生的抗血清，建立了一种用于大鼠生长激素释放因子（rGRF）的异源放射免疫分析方法。根据帕尔科维茨微解剖法检测了大鼠下丘脑中rGRF样免疫反应性（LI）的区域分布，并与生长抑素（SRIF）-LI的分布进行了比较。rGRF和SRIF-LI的含量（平均值±标准误；每毫克蛋白中的纳克数）在正中隆起处最高（rGRF，32.28±11.42；SRIF，109.9±19.2），其次在弓状核中含量丰富（rGRF，3.50±0.47；SRIF，12.88±0.60）。仅在腹内侧核（1.41±0.51）、背内侧核（1.16±0.15）和下丘脑前区（1.29±0.42）中发现少量rGRF-LI，而下丘脑中的其他核团未检测到rGRF-LI。根据其他报道，室周核、腹内侧核、室旁核和下丘脑前区含有相当数量的SRIF-LI。凝胶过滤色谱显示，下丘脑提取物中含有一个与rGRF-(1 - 43)OH相对应的rGRF-LI主峰，以及分别相当于SRIF-(1 - 28)和SRIF-(1 - 14)的两个SRIF-LI峰。这些发现表明，rGRF-LI定位于大鼠的正中隆起和弓状核中，并且rGRF-LI、SRIF-(1 - 28)-LI和SRIF-(1 - 14)-LI在摩尔基础上的比例为1:2.10:6.29。
