Yan Weikai, Falk Duane E
Crop Science Division, Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
Plant Dis. 2002 Dec;86(12):1396-1401. doi: 10.1094/PDIS.2002.86.12.1396.
Effective breeding for disease resistance relies on a thorough understanding of host-by-pathogen relations. Achieving such understanding can be difficult and challenging, particularly for large data sets with complex host genotype-by-pathogen strain interactions. This paper presents a biplot approach that facilitates visual analysis of host-by-pathogen data. A biplot displays both host genotypes and pathogen isolates in a single scatter plot; each genotype or isolate is displayed as a point defined by its scores on the first two principal components derived from subjecting genotype- or strain-centered data to singular value decomposition. From a biplot, clusters of host genotypes and clusters of pathogen strains can be simultaneously visualized. Moreover, the basis for genotype and strain classifications, i.e., interactions between individual genotypes and strains, can be visualized at the same time. A biplot based on genotype-centered data and that based on strain-centered data are appropriate for visual evaluation of susceptibility/resistance of genotypes and virulence/avirulence of strains, respectively. Biplot analysis of genotype-by-strain is illustrated with published response scores of 13 barley line groups to 8 net blotch isolate groups.
有效的抗病育种依赖于对宿主与病原体关系的透彻理解。实现这种理解可能既困难又具有挑战性，尤其是对于具有复杂宿主基因型与病原体菌株相互作用的大型数据集而言。本文提出了一种双标图方法，有助于对宿主与病原体数据进行可视化分析。双标图在单个散点图中展示宿主基因型和病原体分离株；每个基因型或分离株都显示为一个点，该点由其在前两个主成分上的得分定义，这两个主成分来自对以基因型或菌株为中心的数据进行奇异值分解。从双标图中，可以同时可视化宿主基因型簇和病原体菌株簇。此外，基因型和菌株分类的依据，即各个基因型与菌株之间的相互作用，也能同时可视化。基于以基因型为中心的数据的双标图和基于以菌株为中心的数据的双标图分别适用于可视化评估基因型的易感性/抗性和菌株的毒力/无毒力。通过已发表的13个大麦品系组对8个网斑病菌分离株组的反应得分来说明基因型与菌株的双标图分析。
