Bossù Maurizio, Montuori Monica, Casani Daniela, Di Giorgio Gianni, Pacifici Andrea, Ladniak Barbara, Polimeni Antonella
Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, Rome, Italy.
Department DIBAF, Universita degli Studi della Tuscia, Viterbo, Italy.
Int J Paediatr Dent. 2016 Sep;26(5):351-6. doi: 10.1111/ipd.12207. Epub 2015 Oct 6.
Coeliac disease is a chronic small intestinal immune-mediated enteropathy precipitated by exposure to dietary gluten, and possible relationships between coeliac disease and dental pathogenic conditions during childhood have been poorly investigated.
The dental pulp plays a pivotal role in the immune defence against possible entry of pathogens from teeth, and the aim of this work was to investigate quantitative transcription levels of selected genes (IL-9, IL-11, IL-15, IL-18, IL-21, IL-27, MICA, IFN-γ) coding for pro-inflammatory immune innate activities in the pulp of primary teeth from healthy children and children with coeliac disease.
The pulp from primary teeth of 10 healthy children and 10 children with coeliac disease was used to extract RNA and prepare cDNA for quantitative PCR transcription analysis employing commercial nucleotide probes for selected genes.
In children with coeliac disease, the genes coding for pro-inflammatory cytokines IFN-γ, IL-11, IL-18, and IL-21 were significantly overexpressed, suggesting the possible importance of these cytokines in the relationships between coeliac disease and dental disorders.
For the first time, we reported in dental pulp of children possible relationships between coeliac disease and modulation in transcription of cytokine-dependent inflammatory activities.
乳糜泻是一种由摄入膳食麸质引发的慢性小肠免疫介导性肠病，而儿童期乳糜泻与牙齿致病状况之间的潜在关系尚未得到充分研究。
牙髓在抵御病原体从牙齿进入的免疫防御中起关键作用，本研究旨在调查健康儿童和乳糜泻患儿乳牙牙髓中编码促炎免疫固有活性的特定基因（IL-9、IL-11、IL-15、IL-18、IL-21、IL-27、MICA、IFN-γ）的定量转录水平。
选取10名健康儿童和10名乳糜泻患儿的乳牙牙髓，提取RNA并制备cDNA，使用针对特定基因的商业核苷酸探针进行定量PCR转录分析。
在乳糜泻患儿中，编码促炎细胞因子IFN-γ、IL-11、IL-18和IL-21的基因显著过表达，表明这些细胞因子在乳糜泻与牙齿疾病关系中可能具有重要意义。
我们首次在儿童牙髓中报道了乳糜泻与细胞因子依赖性炎症活动转录调节之间的潜在关系。
