Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal.
Centre for Biomedical Research, University of Algarve, Faro, Portugal.
Database (Oxford). 2019 Jan 1;2019. doi: 10.1093/database/baz031.
In response to different stress stimuli, cells transiently form stress granules (SGs) in order to protect themselves and re-establish homeostasis. Besides these important cellular functions, SGs are now being implicated in different human diseases, such as neurodegenerative disorders and cancer. SGs are ribonucleoprotein granules, constituted by a variety of different types of proteins, RNAs, factors involved in translation and signaling molecules, being capable of regulating mRNA translation to facilitate stress response. However, until now a complete list of the SG components has not been available. Therefore, we aimer at identifying and linting in an open access database all the proteins described so far as components of SGs. The identification was made through an exhaustive search of studies listed in PubMed and double checked. Moreover, for each identified protein several details were also gathered from public databases, such as the molecular function, the cell types in which they were detected, the type of stress stimuli used to induce SG formation and the reference of the study describing the recruitment of the component to SGs. Expression levels in the context of different neurodegenerative diseases were also obtained and are also described in the database. The Mammalian Stress Granules Proteome is available at https://msgp.pt/, being a new and unique open access online database, the first to list all the protein components of the SGs identified so far. The database constitutes an important and valuable tool for researchers in this research area of growing interest.
为了应对不同的应激刺激，细胞会短暂地形成应激颗粒（SGs），以保护自身并重新建立平衡。除了这些重要的细胞功能外，SGs 现在还与人类的许多疾病有关，如神经退行性疾病和癌症。SGs 是核糖核蛋白颗粒，由多种不同类型的蛋白质、RNA、参与翻译和信号转导的因子组成，能够调节 mRNA 翻译以促进应激反应。然而，到目前为止，还没有一份完整的 SG 成分清单。因此，我们旨在通过在开放获取数据库中搜索已发表的研究，鉴定并列出迄今为止被描述为 SG 成分的所有蛋白质。通过对 PubMed 中列出的研究进行全面搜索，并进行双重检查，实现了鉴定。此外，对于每个鉴定出的蛋白质，还从公共数据库中收集了一些详细信息，如分子功能、检测到的细胞类型、用于诱导 SG 形成的应激刺激类型以及描述该成分招募到 SGs 的研究的参考信息。还在数据库中描述了在不同神经退行性疾病背景下的表达水平。哺乳动物应激颗粒蛋白组学可在 https://msgp.pt/ 获得，它是一个新的、独特的开放获取在线数据库，是第一个列出迄今为止鉴定出的 SG 所有蛋白成分的数据库。该数据库是该研究领域中日益增长的兴趣的研究人员的重要而有价值的工具。
