基于量子点的高灵敏度荧光猝灭侧向流分析方法的建立及其用于玉米赤霉烯酮的检测。
Development of a high sensitivity quantum dot-based fluorescent quenching lateral flow assay for the detection of zearalenone.
机构信息
Department of Bioengineering, Guangdong Province Engineering Research Center for Antibody Drug and Immunoassay, Jinan University, Guangzhou, 510632, Guangdong, China.
出版信息
Anal Bioanal Chem. 2019 Apr;411(10):2169-2175. doi: 10.1007/s00216-019-01652-1. Epub 2019 Feb 28.
Zearalenone (ZEN) is a common carcinogenic toxin related to cereal contamination. In this study, we developed a high sensitivity quantum dot (QD)-based fluorescent quenching lateral flow assay (LFA) for sensitive ZEN detection. The linear detection range of the fluorescent quenching LFA for ZEN was 0.78-25 ng/mL, and the limit of detection was 0.58 ng/mL. In addition, the fluorescent quenching LFA showed high recovery (83.1-93.6%) for detection of ZEN concentrations spiked into corn samples. These results indicate that the QD-based fluorescent quenching LFA may be a valuable tool for preliminary screening of ZEN contamination.
玉米赤霉烯酮（ZEN）是一种与谷物污染有关的常见致癌毒素。在本研究中，我们开发了一种基于量子点（QD）的高灵敏度荧光猝灭侧向流动分析（LFA）方法，用于灵敏检测 ZEN。荧光猝灭 LFA 检测 ZEN 的线性检测范围为 0.78-25ng/mL，检测限为 0.58ng/mL。此外，荧光猝灭 LFA 对玉米样品中添加的 ZEN 浓度的检测回收率较高（83.1-93.6%）。这些结果表明，基于 QD 的荧光猝灭 LFA 可能是 ZEN 污染初步筛选的有用工具。
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