双荧光免疫层析法同时定量检测玉米样品中的桔青霉素和玉米赤霉烯酮。
Dual fluorescent immunochromatographic assay for simultaneous quantitative detection of citrinin and zearalenone in corn samples.
机构信息
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, China.
Department of Plant Biology, Uppsala BioCenter, Linnean Centre for Plant Biology, Swedish University of Agricultural Science (SLU), P.O. Box 7080, SE-75007 Uppsala, Sweden.
出版信息
Food Chem. 2021 Jan 30;336:127713. doi: 10.1016/j.foodchem.2020.127713. Epub 2020 Jul 31.
The presence of multiple mycotoxins in the agricultural products poses a serious threat to the health of humans and animals. Citrinin (CIT) causes slow growth in animals and damages the kidney function. Zearalenone (ZEN) causes chronic poisoning, abnormal functioning and even death in animals. Herein, a dual fluorescent immunochromatographic assay (DF-ICA) based on europium nanoparticles (EuNPs) was developed for the simultaneous detection of CIT and ZEN in the corn samples. After optimization, the limits of detection (LODs), IC and average recoveries for the simultaneous determination of CIT and ZEN were 0.06 and 0.11 ng/mL, 0.35 and 0.76 ng/mL, from 86.3% to 111.6% and from 86.6% to 114.4%, respectively. Moreover, the DF-ICA was validated by high performance liquid chromatography (HPLC) analyses, and a satisfactory consistency was obtained. In brief, this work demonstrates the feasibility of DF-ICA for simultaneous monitoring of CIT and ZEN in the corn samples.
农产品中存在多种霉菌毒素，对人类和动物的健康构成严重威胁。桔青霉素（CIT）可导致动物生长缓慢，并损害肾功能。玉米赤霉烯酮（ZEN）可导致动物慢性中毒、功能异常，甚至死亡。在此，开发了一种基于铕纳米粒子（EuNPs）的双荧光免疫层析分析（DF-ICA），用于同时检测玉米样品中的 CIT 和 ZEN。经过优化，同时测定 CIT 和 ZEN 的检测限（LOD）、IC 和平均回收率分别为 0.06 和 0.11ng/mL、0.35 和 0.76ng/mL，从 86.3%到 111.6%和从 86.6%到 114.4%。此外，DF-ICA 通过高效液相色谱（HPLC）分析进行了验证，得到了令人满意的一致性。总之，这项工作证明了 DF-ICA 用于同时监测玉米样品中 CIT 和 ZEN 的可行性。
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