Meng Haotian, Guo Yuxin, Jin Xiaoye, Chen Chong, Cui Wei, Shi Jianfeng, Wang Xinxin, Liu Ruolin, Zhu Bofeng
Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, 710004, China.
Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi'an Jiaotong University, Xi'an, 710004, China.
Int J Legal Med. 2019 May;133(3):733-743. doi: 10.1007/s00414-019-02028-x. Epub 2019 Feb 28.
Prior to implementing a new kit into application, developmental validation should be conducted to demonstrate the robustness and applicability of the kit. In this study, 24 Y-STR loci from the AGCU Y SUPP STR kit were tested including 11 loci overlapping with other commercial kits (DYS385a/b, DYS635, DYS533, DYS481, DYS549, DYS460, DYS527a/b, DYS522, and DYS444) and 13 new loci (DYS531, DYS630, DYS622, DYS552, DYS510, DYS459a/b, DYS446, DYS443, DYS587, Y-GATA-A10, DYS520, and DYS557). Developmental validation including PCR-related studies, sensitivity, stability, and species specificity studies were conducted. The performance of the kit in genotyping case-type samples was also estimated. The results indicated that the kit is robust, accurate and sensitive and is able to detect male samples without being affected by female samples or other species. Population data were obtained with this kit in Chinese Xibe group as well. Totally 139 different haplotypes were obtained from 167 male samples and demonstrated that this typing system is relatively discriminative.
在将新试剂盒应用于实际检测之前，应进行开发验证以证明该试剂盒的稳健性和适用性。在本研究中，对AGCU Y SUPP STR试剂盒中的24个Y-STR基因座进行了检测，其中包括11个与其他商业试剂盒重叠的基因座（DYS385a/b、DYS635、DYS533、DYS481、DYS549、DYS460、DYS527a/b、DYS522和DYS444）以及13个新基因座（DYS531、DYS630、DYS622、DYS552、DYS510、DYS459a/b、DYS446、DYS443、DYS587、Y-GATA-A10、DYS520和DYS557）。进行了包括PCR相关研究、灵敏度、稳定性和物种特异性研究在内的开发验证。还评估了该试剂盒在基因分型案例样本中的性能。结果表明，该试剂盒稳健、准确且灵敏，能够检测男性样本，不受女性样本或其他物种的影响。使用该试剂盒在中国锡伯族群体中也获得了群体数据。从167个男性样本中总共获得了139种不同的单倍型，表明该分型系统具有较高的鉴别能力。
