Gulliksson H, Chmielewska J, Gullbring B, Blombäck M
Thromb Res. 1985 Oct 1;40(1):69-79. doi: 10.1016/0049-3848(85)90351-2.
Preservation of red blood cells in special media, such as saline-adenine-glucose-mannitol (SAGM) solution involves storage at a citrate and plasma protein concentration much lower than found in normal CPD or CPD-adenine plasma. In the present study, the effects on the coagulation and other connected enzymatic systems in SAGM medium have been investigated. Significantly higher levels of fibrinopeptide A (FPA) and plasma kallikrein activity were observed in the SAGM medium compared to CPD plasma, indicating an increased enzymatic activation in the SAGM medium. For this reason storage of red cells in SAGM solution, supplemented with citrate, was tested and compared with storage in SAGM and CPD plasma. The results demonstrate a positive effect of citrate as an added ingredient in the SAGM solution. Significantly lower levels of primarily FPA but also decreased activities of plasma kallikrein and general proteolysis were noticed. The decreased citrate concentration in Red Blood Cells stored in SAGM protein poor medium must be considered to have a destabilizing influence on the coagulation and on other connected enzymatic systems.
在特殊培养基（如生理盐水 - 腺嘌呤 - 葡萄糖 - 甘露醇（SAGM）溶液）中保存红细胞，需要在柠檬酸盐和血浆蛋白浓度远低于正常CPD或CPD - 腺嘌呤血浆的条件下储存。在本研究中，已对SAGM培养基中对凝血及其他相关酶系统的影响进行了研究。与CPD血浆相比，在SAGM培养基中观察到纤维蛋白肽A（FPA）水平和血浆激肽释放酶活性显著更高，表明SAGM培养基中的酶激活增加。因此，测试了在添加柠檬酸盐的SAGM溶液中储存红细胞，并与在SAGM和CPD血浆中储存进行比较。结果证明柠檬酸盐作为SAGM溶液中的添加成分具有积极作用。主要观察到FPA水平显著降低，同时血浆激肽释放酶活性和一般蛋白水解活性也降低。必须考虑到储存在低蛋白SAGM培养基中的红细胞中柠檬酸盐浓度降低，会对凝血及其他相关酶系统产生不稳定影响。
