The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
Department of Prosthodontics, Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
Clin Implant Dent Relat Res. 2019 Apr;21(2):324-335. doi: 10.1111/cid.12738. Epub 2019 Mar 1.
Mechanical and biological complications associated with implant systems happen frequently in the clinic.
To develop a chitosan (CS)-based thermosensitive hydrogel for sealing and lubricating purposes in dental implant system.
In this study, a thermosensitive hydrogel made up of CS, β-glycerophosphate pentahydrate (β-GP), and povidone-iodine (PVP-I), called CS/β-GP/PVP-I thermosensitive hydrogel, was fabricated. Three experimental groups with different volume ratios of CS to β-GP were prepared, namely 16/4, 13/7, and 10/10 groups. The surface topography of the different groups and their physicochemical characteristics were examined by SEM, FTIR, and X-ray diffraction analysis. The cytotoxicity of the hydrogel was examined by CCK-8 test. In vitro antibacterial efficiency was analyzed by the spread plate method. Sealing ability was detected by incubating two-piece implants in Escherichia coli suspension. Lubricating ability of the hydrogel was evaluated by the removal torque test with a calibrated digital torque meter.
The CS/β-GP/PVP-I thermosensitive hydrogel was fabricated and showed a highly porous structure under SEM. An in vitro cytotoxicity test demonstrated that 13/7 group displayed no cytotoxicity. Furthermore, all three groups showed obviously antibacterial effects. In the sealing ability test, 16/4 group showed the best sealing ability. The removal torque of 16/4 group and 13/7 group was significantly greater than control group.
Based on our findings, it could be concluded that the thermosensitive and antibacterial CS/β-GP/PVP-I hydrogel with sealing and lubricating ability was successfully prepared. The hydrogel had better sealing and lubricating effects when the volume ratio of CS to β-GP was 16/4.
与植入系统相关的机械和生物并发症在临床上经常发生。
开发一种壳聚糖（CS）基温敏水凝胶，用于牙科植入系统的密封和润滑。
在这项研究中，制备了一种由 CS、β-甘油磷酸五水合物（β-GP）和聚维酮碘（PVP-I）组成的温敏水凝胶，称为 CS/β-GP/PVP-I 温敏水凝胶。制备了三个具有不同 CS 与 β-GP 体积比的实验组，分别为 16/4、13/7 和 10/10 组。通过 SEM、FTIR 和 X 射线衍射分析来检查不同组的表面形貌和理化特性。通过 CCK-8 试验来检测水凝胶的细胞毒性。通过平板法分析体外抗菌效率。通过将两件式植入物在大肠杆菌悬浮液中孵育来检测密封能力。通过使用校准的数字扭矩计评估水凝胶的润滑能力。
制备了 CS/β-GP/PVP-I 温敏水凝胶，在 SEM 下显示出高度多孔的结构。体外细胞毒性试验表明 13/7 组无细胞毒性。此外，三组均表现出明显的抗菌效果。在密封能力测试中，16/4 组显示出最佳的密封能力。16/4 组和 13/7 组的去除扭矩明显大于对照组。
根据我们的发现，可以得出结论，成功制备了具有热致和抗菌性的 CS/β-GP/PVP-I 水凝胶，具有密封和润滑能力。当 CS 与 β-GP 的体积比为 16/4 时，水凝胶具有更好的密封和润滑效果。
