Castro-Arnau Júlia, Marín Ainoa, Castells Marc, Ferrer Iamil, Maestro José L
Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain.
Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain.
J Insect Physiol. 2019 Apr;114:57-67. doi: 10.1016/j.jinsphys.2019.02.010. Epub 2019 Feb 26.
In insects, the insulin receptor (InR) pathway is involved in regulating key physiological processes, including juvenile hormone (JH) synthesis, vitellogenin production, and oocyte growth. This raises the question about which ligand (or ligands) binds to InR to trigger the above effects. We have cloned seven insulin-like peptides (BgILP1 to 7) from female Blattella germanica cockroaches and found that the brain expresses BgILP1 to 6, the fat body BgILP7, and the ovary BgILP2. Starvation induces the reduction of BgILP3, 5, and 6 mRNA levels in the brain, and the various BgILPs are differentially expressed during the gonadotrophic cycle. In addition, by knocking down the BgILPs we were able to identify compensatory regulation at transcriptional level between the different BgILPs, although none of the BgILP knockdown assays, including the knockdown of the seven BgILPs, produced the same phenotypes that we achieved by depleting InR. Taken together, the results indicate that B. germanica ILPs are differentially expressed in tissues and in response to physiological conditions, and that they are affected by compensatory regulation.
在昆虫中，胰岛素受体（InR）信号通路参与调节关键生理过程，包括保幼激素（JH）合成、卵黄原蛋白产生和卵母细胞生长。这就引发了一个问题，即哪种配体（或哪些配体）与InR结合以触发上述效应。我们从雌性德国小蠊蟑螂中克隆了七种胰岛素样肽（BgILP1至7），发现大脑表达BgILP1至6，脂肪体表达BgILP7，卵巢表达BgILP2。饥饿会导致大脑中BgILP3、5和6的mRNA水平降低，并且在促性腺周期中各种BgILP的表达存在差异。此外，通过敲低BgILP，我们能够确定不同BgILP之间在转录水平上的补偿调节，尽管包括敲低七种BgILP在内的所有BgILP敲低实验都没有产生与我们通过耗尽InR所获得的相同表型。综上所述，结果表明德国小蠊的ILP在组织中以及对生理条件的反应中存在差异表达，并且它们受到补偿调节的影响。
