Forster R L, Seifers D L, Strausbaugh C A, Jensen S G, Ball E M, Harvey T L
University of Idaho Research & Extension Center, Kimberly 83341.
Kansas State University Agricultural Research Center - Hays, Hays 67601.
Plant Dis. 2001 Jul;85(7):696-699. doi: 10.1094/PDIS.2001.85.7.696.
The High Plains virus (HPV), which infects corn and other cereals, was first found in 1993 in the United States. Research was initiated in 1995 to investigate the potential for seed transmission of HPV. Sweet corn seeds of various cultivars harvested in 1994 to 1996 from 13 fields and research plots in southwestern Idaho, Colorado, and Nebraska were seeded in potting mix in the greenhouse. Leaf samples collected at the three- to six-leaf stage from both symptomatic and asymptomatic plants were tested by enzyme-linked immunosorbent assay (ELISA). Of the 46,600 seeds planted, 38,473 seedlings emerged, and three tested positive by ELISA, exhibited mosaic symptoms, and had the presence of HPV confirmed by an additional test. One of the positive plants was used for successful acquisition and transmission of HPV by the wheat curl mite to Westford barley. The other two plants were used to successfully transfer HPV to other corn plants by vascular puncture inoculation of seed. These results indicate that HPV can be seed transmitted at a very low frequency in sweet corn.
高平原病毒（HPV）可感染玉米和其他谷类作物，1993年首次在美国被发现。1995年启动了一项研究，以调查HPV通过种子传播的可能性。1994年至1996年从爱达荷州西南部、科罗拉多州和内布拉斯加州的13块田地和研究地块收获的各种甜玉米品种的种子，被播种在温室的盆栽混合料中。在三叶至六叶期从有症状和无症状植株上采集的叶片样本，通过酶联免疫吸附测定（ELISA）进行检测。在种植的46600粒种子中，有38473株幼苗出土，其中3株ELISA检测呈阳性，表现出花叶症状，经进一步检测证实存在HPV。其中一株阳性植株被用于小麦曲叶螨成功获取HPV并将其传播给韦斯特福德大麦。另外两株植株被用于通过种子的维管束穿刺接种成功地将HPV转移到其他玉米植株上。这些结果表明，HPV在甜玉米中能够以极低的频率通过种子传播。
