Im T, Hintz G
Arzneimittelforschung. 1986;36(1):124-6.
The method of determination of factor VIII coagulation antigen (VIIICAg) here described is based on the well established factor VIII inhibitor test. Highly concentrated inhibitors to factor VIII in factor VIII substituted hemophiliacs are the most frequent among inhibitors to clotting factors. If such an inhibitor is used in an inhibitor assay its reaction depends on the VIIICAg concentration in the test system. The slope of the reaction curve is proportional to VIIICAg if the inhibitor titer is known. VIIICAg was measured in one cryoprecipitate and 5 different factor VIII concentrates of medium to high purity using this assay. In addition factor VIII related antigen and factor VIII ristocetin cofactor were determined by routine methods. VIIICAg fairly corresponded to factor VIII coagulation activity. Only cryoprecipitate contained significantly more VIIICAg.
本文所述的凝血因子VIII凝血抗原（VIIICAg）的测定方法基于成熟的凝血因子VIII抑制物检测。在凝血因子抑制物中，凝血因子VIII替代型血友病患者体内高浓度的凝血因子VIII抑制物最为常见。如果在抑制物检测中使用此类抑制物，其反应取决于测试系统中的VIIICAg浓度。若抑制物效价已知，反应曲线的斜率与VIIICAg成正比。使用该检测方法对一份冷沉淀和5种不同中高纯度的凝血因子VIII浓缩物进行了VIIICAg测定。此外，通过常规方法测定了凝血因子VIII相关抗原和凝血因子VIII瑞斯托霉素辅因子。VIIICAg与凝血因子VIII凝血活性相当。只有冷沉淀中含有显著更多的VIIICAg。
