采用两种使用不同抗体的免疫放射分析（IRMA）方法测定血友病中的凝血因子 VIII 凝血抗原（VIIICAg），以及通过 IRMA 测定促凝血因子 VIII（VIIIC）的抑制剂。

Factor VIII clotting antigen (VIIICAg) in haemophilia measured by two immunoradiometric assays (IRMA) using different antibodies, and the measurement of inhibitors to procoagulant factor VIII (VIIIC) by IRMA.

作者信息

Furlong R A, Peake I R, Bloom A L

出版信息

Br J Haematol. 1981 Aug;48(4):643-50. doi: 10.1111/j.1365-2141.1981.00643.x.

DOI:10.1111/j.1365-2141.1981.00643.x

Abstract

Factor VIII clotting antigen (VIIICAg) was measured by immunoradiometric assay (IRMA) using two different antibodies. Both antibodies arose in polytransfused severe haemophiliacs and had similar titres against VIIIC. In 12 normal plasmas there was no significant difference in VIIICAg values obtained (VIIICAg (AbI) = VIIICAg (AbII)). In the majority of 15 severe haemophiliacs tested VIIICAg was undetectable by both antibodies. In 28 mild to moderate haemophiliacs VIIICAg (AbII) was significantly greater than VIIICAg (AbI) (P less than 0.01) suggesting different antigenic determinants. The difference, however, was small and does not affect diagnosis of haemophilia. A modified IRMA has been used to measure anti VIIIC inhibitors by competition of the inhibitor with 125I labelled VIIICAg antibodies for common antigenic determinants. Using an inhibitor of 225 Bethesda units as a standard, results by IRMA of inhibitors in severe haemophiliacs have been similar to those obtained by clotting assay, but with a sensitivity of 0.01 u/ml suggesting the possible use in the detection of weak inhibitors.

摘要

采用两种不同抗体，通过免疫放射分析（IRMA）测定凝血因子 VIII 凝血抗原（VIIICAg）。两种抗体均产生于多次输血的重度血友病患者，且针对 VIIIC 的滴度相似。在 12 份正常血浆中，所测得的 VIIICAg 值无显著差异（VIIICAg（抗体 I）= VIIICAg（抗体 II））。在接受检测的 15 例重度血友病患者中，大多数患者的 VIIICAg 用两种抗体均检测不到。在 28 例轻至中度血友病患者中，VIIICAg（抗体 II）显著高于 VIIICAg（抗体 I）（P 小于 0.01），提示存在不同的抗原决定簇。然而，差异较小，不影响血友病的诊断。一种改良的 IRMA 已用于通过抑制剂与 125I 标记的 VIIICAg 抗体竞争共同抗原决定簇来检测抗 VIIIC 抑制剂。以 225 贝塞斯达单位的抑制剂为标准，重度血友病患者中抑制剂的 IRMA 检测结果与凝血分析结果相似，但其灵敏度为 0.01 u/ml，表明可能用于检测弱抑制剂。