山茱萸新苷通过 SIRT1 介导的 NF-κB 和 FOXO1 信号通路减轻 LPS 诱导的 RAW264.7 细胞炎症。
Sweroside Alleviated LPS-Induced Inflammation via SIRT1 Mediating NF-κB and FOXO1 Signaling Pathways in RAW264.7 Cells.
机构信息
College of Pharmaceutical Sciences, Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, Southwest University, Chongqing 400715, China.
TAAHC-SWU Medicinal Plant R&D Center, XiZang Agriculture and Animal Husbandry College, Nyingchi, Tibet 860000, China.
出版信息
Molecules. 2019 Mar 1;24(5):872. doi: 10.3390/molecules24050872.
was used as a traditional Chinese medicine for the treatment of rheumatoid arthritis. Sweroside was a main iridoid isolated from . The present study aimed to investigate the anti-inflammatory effect mechanism of sweroside. In RAW264.7 cells induced by lipopolysaccharide (LPS), the abnormal proliferation, the NO content increase, and the downregulated Sirtuin1 (SIRT1) expression were observed. Sweroside could alleviate the inflammation by inhibiting cell proliferation through arresting the cell cycle at the G0/G1 phase, by suppressing pro-inflammatory cytokines and by promoting anti-inflammatory cytokines in LPS-induced RAW264.7 cells. Further mechanism research indicated that sweroside could activate the SIRT1, then suppress the nuclear factor-kappa B (NF-κB) and promote the Forkhead transcription factor O1 (FOXO1) signaling pathways. The present study indicated that sweroside may be the main anti-inflammatory constituent of and a promising candidate for anti-inflammation therapy.
被用作治疗类风湿性关节炎的传统中药。水龙骨叶苷是从水龙骨中分离得到的主要环烯醚萜苷。本研究旨在探讨水龙骨叶苷的抗炎作用机制。在脂多糖（LPS）诱导的 RAW264.7 细胞中，观察到异常增殖、NO 含量增加和 Sirtuin1（SIRT1）表达下调。水龙骨叶苷通过将细胞周期阻滞在 G0/G1 期，抑制促炎细胞因子的产生，促进抗炎细胞因子的产生，从而抑制 LPS 诱导的 RAW264.7 细胞的炎症反应。进一步的机制研究表明，水龙骨叶苷可以激活 SIRT1，从而抑制核因子-κB（NF-κB）并促进叉头转录因子 O1（FOXO1）信号通路。本研究表明，水龙骨叶苷可能是水龙骨的主要抗炎成分，是一种有前途的抗炎治疗候选药物。
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