Key Laboratory of Basic and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Department of Pharmaceutical Analysis and Analytical Chemistry, College of Pharmacy, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin 150081, China.
Molecules. 2023 Aug 7;28(15):5925. doi: 10.3390/molecules28155925.
In this study, we developed an ultra-performance liquid chromatography-electrospray tandem quadrupole mass spectrometry (UHPLC-ESI-MS/MS) method to simultaneously determine Picroside-I, Picroside-II, Picroside-III, minecoside, and sweroside in rat plasma. The chromatographic column was an ACQUITY UHPLC BEH Amide Column (2.1 × 100 mm, 1.7 µm; Waters, MA, USA), column temperature 40 °C. The mobile phase was 0.1% formic acid aqueous solution-0.1% formic acid acetonitrile solution. The flow rate was 0.4 mL/min. Multiple reaction monitoring (MRM) and negative ion modes were adopted. The results showed that the calibration curves of five compounds in plasma showed good linearity ( > 0.9911) over the studied dose range. The lower limits of quantification (LLOQ) for Picroside-I, Picroside-II, Picroside-III, minecoside, and sweroside were 6.876, 5.193, 5.040, 1.260, and 4.527 ng/mL, respectively. The intra-day and inter-day precision were <15%. The matrix effects ranged from 95.77 to 101.9%. The were 1.1 ± 0.2, 1.1 ± 0.1, 0.8 ± 0.1, 1.0 ± 0.2, and 2.1 ± 0.1 h. This study will be useful in understanding the behavior of drugs in the body and the body's effect on drugs. It also offers theoretical underpinnings and highlights the importance of clinical applications and creating novel drugs.
在这项研究中，我们开发了一种超高效液相色谱-电喷雾串联四极杆质谱（UHPLC-ESI-MS/MS）方法，用于同时测定大鼠血浆中的胡黄连苷 I、胡黄连苷 II、胡黄连苷 III、马里苷和獐牙菜苦苷。色谱柱为 ACQUITY UHPLC BEH 酰胺柱（2.1×100mm，1.7μm；Waters，MA，USA），柱温 40°C。流动相为 0.1%甲酸水溶液-0.1%甲酸乙腈溶液。流速为 0.4mL/min。采用多反应监测（MRM）和负离子模式。结果表明，五种化合物在血浆中的校准曲线在研究剂量范围内具有良好的线性（>0.9911）。胡黄连苷 I、胡黄连苷 II、胡黄连苷 III、马里苷和獐牙菜苦苷的定量下限（LLOQ）分别为 6.876、5.193、5.040、1.260 和 4.527ng/mL。日内和日间精密度均<15%。基质效应范围为 95.77%至 101.9%。t1/2 分别为 1.1±0.2、1.1±0.1、0.8±0.1、1.0±0.2 和 2.1±0.1h。本研究将有助于了解药物在体内的行为和机体对药物的作用。它还为临床应用和新药创制提供了理论基础和重要性。
