Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Jinan, China.
NHC Key Laboratory of Otorhinolaryngology, Shandong University, Jinan, China.
Inflammation. 2021 Oct;44(5):1961-1968. doi: 10.1007/s10753-021-01473-4. Epub 2021 Apr 29.
Sweroside, as one of the main components of Swertia L. in Gentianaceae, has the effect of clearing heat and detoxifying. In previous studies, sweroside has been reported to have anti-inflammatory effect on LPS-induced inflammation by alleviating NF-κB signaling pathway. In this paper, we investigate the anti-inflammatory effects of sweroside by establishing LPS-induced acute lung injury (ALI) model in mice. Experimental results showed that sweroside could reduce the wet-to-dry ratio of the lung and inhibit MPO activity. In addition, it turned out that sweroside reduced pathological changes in lung tissue and the numbers of inflammatory cells. Moreover, sweroside significantly reduced the levels of inflammatory cytokines and down-regulated the NF-κB signaling pathway. And the results demonstrated that sweroside could increase the expression of SIRT1, and the protective effects of sweroside on LPS-induced ALI were reversed by SIRT1 inhibitor EX-527. In conclusion, sweroside can protect LPS-induced ALI mice through inhibiting inflammation.
獐牙菜苦苷作为龙胆科獐牙菜属植物的主要成分之一，具有清热解毒的功效。在之前的研究中，獐牙菜苦苷通过减轻 NF-κB 信号通路已被报道对 LPS 诱导的炎症具有抗炎作用。在本文中，我们通过建立 LPS 诱导的急性肺损伤 (ALI) 小鼠模型来研究獐牙菜苦苷的抗炎作用。实验结果表明，獐牙菜苦苷可降低肺的湿干比并抑制 MPO 活性。此外，它还减少了肺组织的病理变化和炎症细胞的数量。此外，獐牙菜苦苷显著降低了炎症细胞因子的水平，并下调了 NF-κB 信号通路。结果表明，獐牙菜苦苷可以增加 SIRT1 的表达，而 SIRT1 抑制剂 EX-527 逆转了獐牙菜苦苷对 LPS 诱导的 ALI 的保护作用。总之，獐牙菜苦苷可以通过抑制炎症来保护 LPS 诱导的 ALI 小鼠。
