Suda J, Suda T, Kubota K, Ihle J N, Saito M, Miura Y
Blood. 1986 Apr;67(4):1002-6.
We studied the effect of purified interleukin-3 (IL-3) and erythropoietin on colony formation by hemopoietic progenitors in serum-free cultures of spleen cells from 5-fluorouracil (5-FU)-treated mice. In the presence of IL-3 alone, most of the multilineage (three or more lineages) colonies did not contain erythroid cells. However, in the presence of IL-3 and erythropoietin, most of the multilineage colonies contained various numbers of erythroid cells. Replating experiments suggest that IL-3 maintains the growth of the progenitor cells, which could differentiate into erythroid cells. Erythropoietin facilitated the terminal differentiation and amplification of erythroid cells, although it did not sustain the growth of multipotential stem cells. Single-cell transfer experiments demonstrate that IL-3 supported the late stages of differentiation of neutrophils, macrophages, eosinophils, and megakaryocytes in the absence of lineage-specific factors. Therefore, IL-3 supports the differentiation of multilineage hemopoietic progenitors, and the terminal differentiation of most hemopoietic lineages, with the exception of the erythroid lineage.
我们研究了纯化的白细胞介素-3（IL-3）和促红细胞生成素对5-氟尿嘧啶（5-FU）处理小鼠脾细胞无血清培养中造血祖细胞集落形成的影响。仅在IL-3存在的情况下，大多数多系（三个或更多系）集落不含红系细胞。然而，在IL-3和促红细胞生成素存在的情况下，大多数多系集落含有不同数量的红系细胞。再接种实验表明，IL-3维持祖细胞的生长，这些祖细胞可分化为红系细胞。促红细胞生成素促进红系细胞的终末分化和扩增，尽管它不能维持多能干细胞的生长。单细胞移植实验表明，在缺乏谱系特异性因子的情况下，IL-3支持中性粒细胞、巨噬细胞、嗜酸性粒细胞和巨核细胞分化的后期阶段。因此，IL-3支持多系造血祖细胞的分化，以及除红系谱系外大多数造血谱系的终末分化。
