辛基修饰的中孔泡沫体中脂肪酶交联酶聚集体的制备。
Preparation of lipase cross-linked enzyme aggregates in octyl-modified mesocellular foams.
机构信息
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
出版信息
Int J Biol Macromol. 2019 Jun 1;130:342-347. doi: 10.1016/j.ijbiomac.2019.02.154. Epub 2019 Feb 27.
Lipase r27RCL from Rhizopus chinensis was immobilized onto octyl-modified mesocellular foams (MCFs-C8) via two-step process of enzyme adsorption and cross-linking. Oxidized gum arabic was used as substitute for harmful glutaraldehyde to improve catalytic performance of immobilized enzyme for catalysis in non-aqueous phase. The parameters like aldehyde concentration, cross-linking time were optimized. Cross-linked enzyme aggregates (CLEAs) of lipase r27RCL prepared in MCFs-C8 by using oxidized gum arabic (GA-CLEAs@MCFs-C8) showed the highest esterification activity (145 μmol min mg protein) compared with lipase adsorbed onto MCFs-C8 (MCFs-C8-r27RCL) (98 μmol min mg protein), CLEAs of lipase in MCFs-C8 by glutaraldehyde (G-CLEAs@MCFs-C8) (88 μmol min mg protein) and immobilized lipase onto octyl/epoxy (1,1, v/v) modified MCFs (MCFs-octyl-epoxy-r27RCL) (35 μmol min mg protein). Moreover, GA-CLEAs@MCFs-C8 exhibited excellent thermal and mechanical stability, and could still maintain 69% of initial activity after 5 time cycles.
里氏木霉脂肪酶 r27RCL 通过酶吸附和交联两步法固定在辛基修饰的介孔泡沫（MCFs-C8）上。氧化阿拉伯胶被用作有害戊二醛的替代品，以提高固定化酶在非水相催化中的催化性能。优化了醛浓度、交联时间等参数。与吸附在 MCFs-C8 上的脂肪酶（MCFs-C8-r27RCL）（98μmol min mg 蛋白）相比，用氧化阿拉伯胶制备的 MCFs-C8 中的交联酶聚集体（CLEAs）（GA-CLEAs@MCFs-C8）显示出最高的酯化活性（145μmol min mg 蛋白），固定在 MCFs-C8 中的脂肪酶 CLEAs 用戊二醛（G-CLEAs@MCFs-C8）（88μmol min mg 蛋白）和固定在辛基/环氧（1,1，v/v）修饰的 MCFs 上的脂肪酶（MCFs-octyl-epoxy-r27RCL）（35μmol min mg 蛋白）。此外，GA-CLEAs@MCFs-C8 表现出优异的热稳定性和机械稳定性，在 5 次循环后仍能保持初始活性的 69%。
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