在氧化的海藻酸钠修饰的中孔泡沫上形成脂肪酶交联酶聚集体。
Formation lipase cross-linked enzyme aggregates on octyl-modified mesocellular foams with oxidized sodium alginate.
机构信息
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
出版信息
Colloids Surf B Biointerfaces. 2019 Dec 1;184:110501. doi: 10.1016/j.colsurfb.2019.110501. Epub 2019 Sep 11.
Supported cross-linked enzyme aggregates were prepared by immobilization of Candida antarctica lipase B onto hydrophobic surface of octyl-modified mesocellular foams (MCFs-C8). Oxidized sodium alginate was used as a substitute for traditional glutaraldehyde. Supported cross-linked enzyme aggregates using oxidized sodium alginate (SA-CLEAs@MCFs-C8) exhibited significantly improved thermal stability and organic solvents tolerance compared to the free lipase, lipase adsorbed onto MCFs-C8 and supported cross-linked enzyme aggregates using glutaraldehyde (G-CLEAs@MCFs-C8). Then immobilized lipases were employed for biodiesel production by transesterification of soybean oil with methanol. In the optimization condition, SA-CLEAs@MCFs-C8 were quite stable and still showed high fatty acid methyl esters (FAME) yield after 5 repeated cycles (from 89% to 78%), whereas MCFs-C8-CALB retained 67% FAME yield (about 72% for G-CLEAs@MCFs-C8).
通过将南极假丝酵母脂肪酶 B 固定在辛基修饰的介孔泡沫（MCFs-C8）的疏水面上，制备了支持的交联酶聚集体。用过氧化化的海藻酸钠代替传统的戊二醛。与游离脂肪酶、吸附在 MCFs-C8 上的脂肪酶和用戊二醛（G-CLEAs@MCFs-C8）制备的支持的交联酶聚集体相比，用过氧化化的海藻酸钠（SA-CLEAs@MCFs-C8）制备的支持的交联酶聚集体表现出显著提高的热稳定性和有机溶剂耐受性。然后，通过大豆油与甲醇的酯交换反应，将固定化脂肪酶用于生物柴油的生产。在优化条件下，SA-CLEAs@MCFs-C8 非常稳定，在 5 次重复循环后（从 89%降至 78%）仍显示出高脂肪酸甲酯（FAME）产率，而 MCFs-C8-CALB 保留了 67%的 FAME 产率（G-CLEAs@MCFs-C8 约为 72%）。
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