来自印度加尔各答的高粘滞性多粘菌素耐药肺炎克雷伯菌：基因组和表型分析。
Hypermucoviscous polymyxin-resistant Klebsiella pneumoniae from Kolkata, India: Genomic and phenotypic analysis.
机构信息
School of Health & Life Sciences, Federation University Australia, P.O. Box 663, Ballarat, VIC, Australia.
Clinical Microbiology & Hospital Infection Control, Belle Vue Clinic, 9U N Brahmachari Street, Kolkata 17, India.
出版信息
J Glob Antimicrob Resist. 2019 Jun;17:1-2. doi: 10.1016/j.jgar.2019.02.012. Epub 2019 Feb 27.
OBJECTIVES
Here we report the draft genome sequence of a colistin-resistant hypermucoviscous Klebsiella pneumoniae isolated from a hospitalised patient with acute kidney injury in Kolkata, India.
METHODS
Whole genomic DNA was sequenced using an Illumina HiSeq platform. The generated reads were de novo assembled using SPAdes v.3.7.1. Genome annotation was performed using the NCBI Prokaryote Genome Annotation Pipeline (PGAP) v.4.6. The sequence type (ST), capsular type, antimicrobial resistance and virulence-related genes were predicted from the genome sequence.
RESULTS
Klebsiella pneumoniae KP26 belonged to ST147. The assembly comprised 63 contigs (>1000 bp) with a total read length of 5 560 935 bp and a total of 5399 coding sequences. The isolate was resistant to most β-lactams, aminoglycosides, quinolones, fosfomycin, trimethoprim, sulphonamides and polymyxins. No mcr genes were detected in the genome.
CONCLUSION
Isolate KP26 is a hypermucoviscous, multidrug-resistant K. pneumoniae strain that may represent an emerging high-risk clone associated with severe infections in India.
目的
我们在此报告一株高产黏菌素耐药超黏液型肺炎克雷伯菌的基因组序列草案，该菌从印度加尔各答一名患有急性肾损伤的住院患者中分离得到。
方法
使用 Illumina HiSeq 平台对全基因组 DNA 进行测序。使用 SPAdes v.3.7.1 从头组装生成的reads。使用 NCBI Prokaryote Genome Annotation Pipeline (PGAP) v.4.6 进行基因组注释。从基因组序列中预测序列型（ST）、荚膜型、抗微生物药物耐药性和毒力相关基因。
结果
肺炎克雷伯菌 KP26 属于 ST147。组装由 63 个（>1000 bp）contigs 组成，总读取长度为 5560935 bp，总共有 5399 个编码序列。该分离株对大多数β-内酰胺类、氨基糖苷类、喹诺酮类、磷霉素、甲氧苄啶、磺胺类和黏菌素耐药。基因组中未检测到 mcr 基因。
结论
分离株 KP26 是一株高产黏菌素耐药的超黏液型肺炎克雷伯菌，可能代表与印度严重感染相关的一种新兴高风险克隆。
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