Department of Pathogenic Biology, School of Basic Medicine, Southwest Medical University, No. 319 Zhongshan Road, Luzhou 646000, Sichuan, China; Department of Human Assisted Reproduction Medical and Technology, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan, China.
The People's Hospital of Henan Province, Zhengzhou 450000, Henan, China.
J Glob Antimicrob Resist. 2020 Mar;20:272-274. doi: 10.1016/j.jgar.2020.01.006. Epub 2020 Jan 30.
The aim of this study was to characterise a high biofilm-forming capacity, hypermucoviscous, bla and bla co-producing Klebsiella pneumoniae strain (KSH203).
Antimicrobial susceptibility, biofilm formation and hypermucoviscous phenotype were determined by the disk diffusion method, crystal violet staining and positive string test, respectively. Whole-genome sequencing was performed using a PacBio RS II Sequencer. High-quality reads were de novo assembled using Celera Assembler v.8.0. Genome annotation was performed using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP), and the genome characteristics were analysed by bioinformatics methods.
Klebsiella pneumoniae strain KSH203 was resistant to all antibiotics tested but was only intermediate-resistant to polymyxin B. This strain showed high biofilm-forming ability and a hypermucoviscous phenotype with serotype K25 belonging to the ST11 clone. KSH203 consists of a 5 464 059-bp single chromosome and four plasmids including pKSH203-NDM (53 144 bp), pKSH203-KPC (159 467 bp), pKSH203-CTX-M-3 (156 910 bp) and pKSH203-qnrS (253 705 bp). A total of 44 antimicrobial resistance genes and a large number virulence-associated genes were identified in the genome of strain KSH203.
In this study, we illustrate the whole genome sequence of high biofilm-forming capacity, hypermucoviscous K. pneumoniae isolate KSH203 with capsular serotype K25 belonging to ST11 isolated from a patient in China, which carried a large number of antimicrobial resistance genes and virulence-associated genes. Future studies are needed to be aware of dissemination of this type of strain among environmental, animal and human isolates.
本研究旨在对一株高生物膜形成能力、产超广谱β-内酰胺酶（ESBLs）和碳青霉烯酶的肺炎克雷伯菌（Klebsiella pneumoniae）KSH203 进行特征描述。
采用纸片扩散法、结晶紫染色法和阳性拉丝试验分别测定抗菌药物敏感性、生物膜形成能力和高黏液表型。使用 PacBio RS II 测序仪进行全基因组测序。使用 Celera Assembler v.8.0 对高质量读取序列进行从头组装。使用 NCBI Prokaryotic Genome Annotation Pipeline (PGAP) 进行基因组注释，并通过生物信息学方法分析基因组特征。
肺炎克雷伯菌 KSH203 对所有测试的抗生素均具有耐药性，但对多黏菌素 B 仅表现为中介耐药。该菌株表现出高生物膜形成能力和高黏液表型，血清型为 K25，属于 ST11 克隆。KSH203 由一条 5464059bp 的单染色体和四条质粒组成，包括 pKSH203-NDM（53144bp）、pKSH203-KPC（159467bp）、pKSH203-CTX-M-3（156910bp）和 pKSH203-qnrS（253705bp）。在 KSH203 菌株的基因组中，共鉴定出 44 种抗菌药物耐药基因和大量毒力相关基因。
本研究阐明了一株高生物膜形成能力、产超广谱β-内酰胺酶和碳青霉烯酶的肺炎克雷伯菌 KSH203 的全基因组序列，该菌携带大量抗菌药物耐药基因和毒力相关基因，血清型为 K25，属于 ST11 克隆，从中国的一名患者中分离得到。未来的研究需要注意此类菌株在环境、动物和人类分离株中的传播。
