中国产耐替加环素 KPC-2 型肺炎克雷伯菌 ST15 临床分离株的全基因组草图序列
Draft genome sequence of a tigecycline-resistant KPC-2-producing Klebsiella pneumoniae ST15 clinical isolate from China.
机构信息
Institute of Hygiene, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, China.
Institute of Virology, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, China.
出版信息
J Glob Antimicrob Resist. 2017 Dec;11:156-158. doi: 10.1016/j.jgar.2017.10.017. Epub 2017 Oct 31.
OBJECTIVES
Carbapenem-resistant Klebsiella pneumoniae (CRKP) infections have emerged worldwide as a serious challenge to public health. Tigecycline is regarded as one of the last-resort antimicrobials for CRKP infections. Here we report the draft genome sequence of a clinical tigecycline-resistant KPC-2-producing K. pneumoniae isolate (TRKP1).
METHODS
Whole-genome sequencing of strain TRKP1 was performed using an Illumina Hiseq™ X Ten system. The generated reads were assembled using CLC Genomics Workbench. Functional annotation was performed by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) server. Bioinformatics analysis was further performed.
RESULTS
Klebsiella pneumoniae TRKP1 belongs to sequence type ST15, corresponding to clonal complex CC23. The total genome length of the isolate was 5609823bp, harbouring 19 genes relevant to antibiotic resistance. Antimicrobial susceptibility testing revealed that the isolate is resistant to all antibiotics except colistin.
CONCLUSIONS
The genome sequence of K. pneumoniae TRKP1 will facilitate the understanding of drug resistance mechanisms and genomic features of clinically isolated tigecycline-resistant CRKP.
目的
碳青霉烯类耐药肺炎克雷伯菌（CRKP）已在全球范围内出现，成为公共卫生的严重挑战。替加环素被认为是治疗 CRKP 感染的最后手段之一。本研究报告了一株临床分离的产 KPC-2 型替加环素耐药肺炎克雷伯菌（TRKP1）的基因组序列草案。
方法
使用 Illumina Hiseq™ X Ten 系统对 TRKP1 菌株进行全基因组测序。使用 CLC Genomics Workbench 对生成的读数进行组装。通过 NCBI Prokaryotic Genome Annotation Pipeline（PGAP）服务器进行功能注释。进一步进行生物信息学分析。
结果
肺炎克雷伯菌 TRKP1 属于序列型 ST15，对应克隆群 CC23。该分离株的总基因组长度为 5609823bp，包含 19 个与抗生素耐药性相关的基因。药敏试验显示，该分离株除黏菌素外，对所有抗生素均耐药。
结论
肺炎克雷伯菌 TRKP1 的基因组序列将有助于理解临床分离的替加环素耐药 CRKP 的耐药机制和基因组特征。
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