用于检测自然感染猪体内胞内劳森菌暴露情况的口腔液。

Oral fluid for detection of exposure to Lawsonia intracellularis in naturally infected pigs.

作者信息

Barrera-Zarate Javier A, Andrade Mariana R, Pereira Carlos E R, Vasconcellos Amanda, Wagatsuma Mariane M, Sato Jose P H, Daniel Amanda Gabrielle S, Rezende Lucas A, Otoni Luisa A V, Laub Ricardo P, Macedo Nubia R, Costa Camila M, Haddad João Paulo A, Guedes Roberto M C

机构信息

Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil.

出版信息

Vet J. 2019 Feb;244:34-36. doi: 10.1016/j.tvjl.2018.12.003. Epub 2018 Dec 7.

DOI:10.1016/j.tvjl.2018.12.003

PMID:30825892

Abstract

To demonstrate the utility of oral fluid (OF) for indirect diagnostic detection of Lawsonia intracellularis (Li), 15 pig farms were studied. Serum and fecal samples were collected from 20 animals from five different age groups on each farm. OF samples were collected from animals in two pens of the same age groups. Serum and OF samples were analyzed in an immunoperoxidase in monolayer assay (IPMA) for the detection of anti-Li immunoglobulin G (IgG) and A (IgA). Compatible results were found between PCR and IgG in OF in four of the five ages evaluated. Simultaneous detection of IgG in serum and OF was mainly observed on farms showing clinical signs suggestive of porcine proliferative enteropathy (PPE). These findings demonstrate the potential usefulness of OF in detecting anti-Li antibodies as a diagnostic tool that can be used to monitor PPE in herds with clinical signs compatible with the disease.

摘要

为证明口腔液（OF）用于间接诊断检测细胞内劳森菌（Li）的效用，对15个猪场进行了研究。从每个猪场五个不同年龄组的20只动物采集血清和粪便样本。从相同年龄组的两个猪栏中的动物采集OF样本。血清和OF样本采用单层免疫过氧化物酶测定法（IPMA）分析，以检测抗Li免疫球蛋白G（IgG）和A（IgA）。在评估的五个年龄中的四个年龄中，PCR与OF中的IgG结果相符。血清和OF中IgG的同时检测主要在表现出提示猪增生性肠炎（PPE）临床症状的猪场中观察到。这些发现证明了OF在检测抗Li抗体方面作为一种诊断工具的潜在用途，可用于监测具有与该疾病相符临床症状的猪群中的PPE。