Holyoake P K, Emery D, Gonsalves J, Donahoo M, Collins A
Industry and Investment NSW, Wagga Wagga Agricultural Institute, Wagga Wagga, New South Wales 2650, Australia.
Aust Vet J. 2010 May;88(5):186-8. doi: 10.1111/j.1751-0813.2010.00558.x.
Proliferative enteropathy (PE) of pigs is caused by Lawsonia intracellularis. Clinical severity appears to depend, at least partly, on the infective dose and strain of L. intracellularis. Serological tests are able to detect subclinical disease. The Bioscreen ELISA for detecting L. intracellularis-specific antibodies is widely used to monitor the circulating antibody status of pigs in Australia, but its sensitivity and specificity have not been reported. The aim of the present study was to measure the seroprevalence of antibodies to L. intracellularis in growing pigs in Australia.
Test sera were sourced from 1817 serum samples collected from finisher pigs from 63 herds across Australia in 2001, selected from a larger sample of 180 herds to represent the contribution that each herd size makes to the number of pigs produced. The test sera were the most recent collection of pig sera from all states and samples had been stored at -80 degrees C from 2001 until testing was conducted in 2008. Sera were tested using the BioScreen ELISA.
All herds tested positive for L. intracellularis-specific antibodies. The mean percentage of positive samples within each herd was 84.2% (range 31.3-100%).
Lawsonia intracellularis is endemic in pig herds in Australia and cost-effective strategies to reduce reliance on antibiotics, such as vaccination and/or all-in/all-out pig flow coupled with cleaning and disinfection of pens, are warranted.
猪增生性肠炎（PE）由胞内劳森菌引起。临床严重程度似乎至少部分取决于胞内劳森菌的感染剂量和菌株。血清学检测能够检测亚临床疾病。用于检测胞内劳森菌特异性抗体的Bioscreen ELISA在澳大利亚被广泛用于监测猪的循环抗体状态，但其敏感性和特异性尚未见报道。本研究的目的是测定澳大利亚生长猪中胞内劳森菌抗体的血清流行率。
检测血清来自2001年从澳大利亚63个猪群的育肥猪采集的1817份血清样本，这些猪群是从180个猪群的较大样本中挑选出来的，以代表每个猪群规模对生猪产量的贡献。检测血清是来自所有州的最新猪血清样本，样本自2001年起一直保存在-80℃，直至2008年进行检测。使用Bioscreen ELISA检测血清。
所有检测的猪群胞内劳森菌特异性抗体均呈阳性。每个猪群内阳性样本的平均百分比为84.2%（范围31.3 - 100%）。
胞内劳森菌在澳大利亚的猪群中呈地方性流行，有必要采取经济有效的策略来减少对抗生素的依赖，如接种疫苗和/或采用全进全出的猪群流动方式并结合猪舍的清洁和消毒。
