一种用于猪增生性肠炎血清学诊断的新型酶联免疫吸附测定法的评估

Evaluation of a novel enzyme-linked immunosorbent assay for serological diagnosis of porcine proliferative enteropathy.

作者信息

Boesen Henriette Toft, Jensen Tim Kåre, Møller Kristian, Nielsen Lisbeth Harm, Jungersen Gregers

机构信息

Danish Institute for Food and Veterinary Research, Bülowsvej 27, DK-1790 Copenhagen V, Denmark.

出版信息

Vet Microbiol. 2005 Aug 10;109(1-2):105-12. doi: 10.1016/j.vetmic.2005.05.004.

DOI:10.1016/j.vetmic.2005.05.004

PMID:15975740

Abstract

A specific enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the porcine pathogen Lawsonia intracellularis was developed and evaluated using sera from naïve, naturally infected as well as experimentally infected pigs. On the basis of 37 serum samples collected from experimentally infected pigs and 62 serum samples from naturally infected pigs the sensitivity of the ELISA was calculated to 98.0%. The specificity of the test was 99.3%, calculated on the basis of 273 serum samples collected in six herds free of L. intracellularis after medicated eradication. The novel ELISA was a specific and sensitive method for detecting specific antibodies, and may be a good alternative to the existing serological tests for L. intracellularis. It may be usable for diagnosis of proliferative enteropathy and for determination of a herd's epidemiologic status.

摘要

开发了一种用于检测猪胞内劳森菌病原体抗体的特异性酶联免疫吸附测定（ELISA），并使用来自未接触过病原体、自然感染以及实验感染猪的血清进行了评估。根据从实验感染猪采集的37份血清样本和自然感染猪的62份血清样本，计算出ELISA的敏感性为98.0%。该检测方法的特异性为99.3%，这是根据在六个经药物根除胞内劳森菌的猪群中采集的273份血清样本计算得出的。这种新型ELISA是检测特异性抗体的一种特异性和敏感性均良好的方法，可能是现有胞内劳森菌血清学检测的良好替代方法。它可用于诊断增生性肠炎以及确定猪群的流行病学状况。