Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu Province, 210023, China.
Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu Province, 210023, China.
Microbiol Res. 2019 Apr;221:50-59. doi: 10.1016/j.micres.2019.02.005. Epub 2019 Feb 11.
The endophytic fungus Phomopsis liquidambari play a key role in habitat adaptation of rice (Oryza sativa L.) with potential multiple beneficial. However, our previous published work on this subject remains incomplete. Here, we performed a soil nutrient (nitrogen and phosphorus) transformation with related functional genes and elucidated how rhizosphere microbiota vary their response to P. liquidambari interaction throughout the plant's life cycle under field conditions by Illumina Miseq sequencing platforms in a nutrient-limited paddy soil. Our results showed that P. liquidambari symbiosis decreased the nitrogen and phosphorus loss by 24.59% and 17.46% per pot, respectively. Additionally, we suggest that the application of P. liquidambari altered the activation of soil nitrogen and phosphorus functional genes to accelerate nutrient turnover in the rice rhizosphere. High-throughput sequencing with co-occurrence network and species-related network analysis further revealed that P. liquidambari colonization influenced the patterns of microbiota shift in the rhizosphere, especially during the heading stages. This led to an optimized microbial community through the promotion and inhibition of indigenous soil microbes with a higher level of available nutrient supplies. Our study strongly proposes rice-P. liquidambari symbiosis as a useful candidate for improving N and P acquisition and utilization.
植物内生真菌密粘褶菌在水稻（Oryza sativa L.）的栖息地适应中发挥着关键作用，具有潜在的多种益处。然而，我们之前关于这个主题的发表工作仍然不完整。在这里，我们通过 Illumina Miseq 测序平台，在养分有限的稻田中，在田间条件下，进行了土壤养分（氮和磷）转化及其相关功能基因的研究，并阐明了根际微生物群如何在植物整个生命周期中对密粘褶菌相互作用做出响应。我们的结果表明，密粘褶菌共生体使每盆的氮和磷损失分别减少了 24.59%和 17.46%。此外，我们认为密粘褶菌的应用改变了土壤氮和磷功能基因的激活，从而加速了水稻根际的养分周转。共现网络和物种相关网络分析的高通量测序进一步揭示了密粘褶菌定殖影响了根际微生物区系变化的模式，特别是在抽穗期。这通过促进和抑制具有更高可用养分供应水平的土著土壤微生物，优化了微生物群落。我们的研究强烈提出水稻-密粘褶菌共生体是提高氮磷吸收和利用的一个有用候选体。
