Jing Minyu, Xu Xihui, Peng Jing, Li Can, Zhang Hanchao, Lian Chunlan, Chen Yahua, Shen Zhenguo, Chen Chen
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Asian Research Center for Bioresource and Environmental Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Midori-cho, Tokyo 188-0002, Japan.
J Fungi (Basel). 2022 Jun 29;8(7):690. doi: 10.3390/jof8070690.
includes both plant pathogenic and beneficial fungi. Although endophytes beneficial to plants have high potential for plant growth promotion and improving stress tolerance, studies on endophytic lifestyles and endophyte-plant interactions are still limited. Here, three endophytes belonging to , AS31, AS33, and AS42, were isolated. They could successfully colonize rice roots and significantly improved rice growth. The genomes of strains AS31, AS33, and AS42 were sequenced and compared with other species covering both pathogens and endophytes. The genomes of AS31, AS33, and AS42 were 36.8, 34.8, and 35.3 Mb, respectively. The endophytic genomes had more genes encoding carbohydrate-active enzymes (CAZymes) and small secreted proteins (SSPs) and secondary metabolism gene clusters involved in indole metabolism than the pathogens. In addition, these endophytes were able to improve Pi (phosphorus) accumulation and transport in rice by inducing the expression of Pi transport genes in rice. Specifically, inoculation with endophytes significantly increased Pi contents in roots at the early stage, while the Pi contents in inoculated shoots were significantly increased at the late stage. Our results not only provide important insights into endophyte-plant interactions but also provide strain and genome resources, paving the way for the agricultural application of endophytes.
包括植物病原真菌和有益真菌。虽然对植物有益的内生菌在促进植物生长和提高抗逆性方面具有很高的潜力，但关于内生菌生活方式和内生菌与植物相互作用的研究仍然有限。在此，分离出了属于AS31、AS33和AS42的三种内生菌。它们能够成功定殖于水稻根部，并显著促进水稻生长。对AS31、AS33和AS42菌株的基因组进行了测序，并与涵盖病原体和内生菌的其他物种进行了比较。AS31、AS33和AS42的基因组大小分别为36.8、34.8和35.3 Mb。与病原体相比，内生菌基因组具有更多编码碳水化合物活性酶（CAZymes）、小分泌蛋白（SSPs）以及参与吲哚代谢的次生代谢基因簇。此外，这些内生菌能够通过诱导水稻中磷转运基因的表达来提高水稻对磷（Pi）的积累和转运。具体而言，接种内生菌在早期显著增加了根部的磷含量，而在后期接种植株地上部分的磷含量显著增加。我们的研究结果不仅为内生菌与植物的相互作用提供了重要见解，还提供了菌株和基因组资源，为内生菌在农业上的应用铺平了道路。
