Jeon Chang-Wook, Kim Da-Ran, Bae Eun-Ji, Kwak Youn-Sig
Dvision of Applied Life Science (BK21 Plus) and Research Institute of Life Sciences, Gyeongsang National University, Jinju, South Korea.
Forest Biomaterials Research Center, National Institute of Forest Science, Jinju, South Korea.
Front Bioeng Biotechnol. 2021 Jan 15;8:530067. doi: 10.3389/fbioe.2020.530067. eCollection 2020.
There is increasing attention being paid to utilizing microbial communities to improve plant health while reducing management inputs. Thus, the objectives of this research were to assess changes in the rhizosphere bacterial community structure associated with long-term turfgrass monoculture and to demonstrate the feasibility of using functional bacteria as beneficial biocontrol agents. Large patch disease, caused by the fungal pathogen AG2-2, is a significant threat to turfgrass cultivation. Rhizosphere samples were collected from 2-, 13- and 25-year turfgrass () monocultures. The 13-year monoculture field had a higher pathogen population density than both the 2- and 25-year monoculture fields. Analyses of the rhizosphere bacterial communities revealed that was dominant in the 2-year field and was enriched in the 25-year field. Based on the culturable rhizosphere bacteria, J6 and J10 were obtained from the 2- and 25-year fields, respectively. Application of J6 and J10 led to excellent inhibition of large patch disease as well as enhanced tolerance against drought and temperature stresses. The results showed that the selected bacteria could be developed as biocontrol and abiotic stress tolerance agents for turfgrass cultivation.
人们越来越关注利用微生物群落来改善植物健康状况，同时减少管理投入。因此，本研究的目的是评估与长期草坪草单一栽培相关的根际细菌群落结构变化，并证明使用功能细菌作为有益生物防治剂的可行性。由真菌病原体AG2-2引起的大片病是草坪草种植的重大威胁。从2年、13年和25年的草坪草单一栽培中采集根际样本。13年单一栽培田的病原菌种群密度高于2年和25年单一栽培田。对根际细菌群落的分析表明， 在2年田占主导地位，而 在25年田富集。基于可培养的根际细菌，分别从2年田和25年田获得了J6和J10。施用J6和J10对大片病有极好的抑制作用，同时增强了对干旱和温度胁迫的耐受性。结果表明，所选细菌可开发为草坪草种植的生物防治和非生物胁迫耐受性剂。
