CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences(CAS), Shandong Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai, Shandong, 264003, PR China.
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences(CAS), Shandong Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai, Shandong, 264003, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
Environ Pollut. 2019 May;248:462-470. doi: 10.1016/j.envpol.2019.02.062. Epub 2019 Feb 25.
The abuse of antibiotics has caused the propagation of antibiotic resistance genes (ARGs) in aquaculture systems. Although the recirculating systems have been considered as a promising approach for preventing the coastal water pollution of antibiotics and ARG, rare information is available on the distribution and proliferation of ARGs in the recirculating mariculture system. This study firstly investigated the proliferation of ARGs in coastal recirculating mariculture systems. Ten subtypes of ARGs including tet (tetB, tetG, tetX), sul (sul1, sul2), qnr (qnrA, qnrB, qnrS), and erm (ermF, ermT) were detected. The absolute abundances of the ARGs detected in the mariculture farm were more than 1 × 10 copies/mL. The sulfonamide resistance genes (sul1 and sul2) were the most abundant ARGs with the abundance of 3.5 × 10-6.5 × 10 copies/mL. No obvious correlation existed between the antibiotics and ARGs. Some bacteria were positively correlated with two or more ARGs to indicate the occurrence of multidrug resistance. The fluidized-bed biofilter for wastewater treatment in the recirculating system was the main breeding ground for ARGs while the UV sterilization process could reduce the ARGs. The highest flux of ARGs (6.5 × 10 copies/d) indicated that the discharge of feces and residual baits was the main gateway for ARGs in the recirculating mariculture system to enter the environments.
抗生素的滥用导致了水产养殖系统中抗生素耐药基因（ARGs）的传播。虽然循环系统被认为是防止抗生素和 ARG 污染沿海水的一种有前途的方法，但关于循环海水养殖系统中 ARG 的分布和增殖的信息很少。本研究首先调查了沿海循环海水养殖系统中 ARG 的增殖情况。检测到包括 tet（tetB、tetG、tetX）、sul（sul1、sul2）、qnr（qnrA、qnrB、qnrS）和 erm（ermF、ermT）在内的 10 种 ARG 亚型。养殖农场中检测到的 ARG 的绝对丰度超过 1×10 拷贝/mL。磺胺类耐药基因（sul1 和 sul2）是最丰富的 ARG，丰度为 3.5×10-6 至 5×10-6 拷贝/mL。抗生素和 ARG 之间没有明显的相关性。一些细菌与两种或更多的 ARG 呈正相关，表明存在多药耐药性。循环系统中用于处理废水的流化床生物滤器是 ARG 的主要滋生地，而紫外线消毒过程可以减少 ARG。ARG 的最高通量（6.5×10 拷贝/d）表明，粪便和残余诱饵的排放是循环海水养殖系统中 ARG 进入环境的主要途径。
