CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, Shandong, 264003, PR China; Shandong Key Laboratory of Coastal Environmental Processes, Yantai, Shandong, 264003, PR China.
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, Shandong, 264003, PR China; Shandong Key Laboratory of Coastal Environmental Processes, Yantai, Shandong, 264003, PR China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266071, China.
Chemosphere. 2022 Aug;301:134718. doi: 10.1016/j.chemosphere.2022.134718. Epub 2022 Apr 26.
The irrational use of antibiotics has given rise to the proliferation of antibiotic resistance genes (ARGs) in coastal bays. There were few reports on the seasonal distribution of ARGs under the influence of land-ocean interaction in coastal bay. This work studied the seasonal and spatial proliferation of ARGs under the influences of land-ocean interaction in the Sishili Bay. Ten ARGs including tetB, tetG, tetX, sul1, sul2, qnrA, qnrB, qnrS, ermF, ermT and class 1 integron-integrase gene (intI1) were detected and quantified. The relative abundances of intI1 and most of ARGs were in orders of magnitude of 1 × 10-2 copies/16S rRNA copies. The abundances of total ARGs in autumn and summer were much higher than those in the other seasons. Estuary, port and aquaculture farms were important reservoirs of ARGs in the bay. The nutrient levels in coastal water were positively associated with most of the ARGs and intI1, indicating that the water quality was an important driver of ARGs and their transmission. The land-based discharge and seawater stratification were proved to be the dominant driving factors for the seasonal distribution of ARGs in the coastal bay. The land-based discharge and seawater stratification were enhanced from spring to summer, which led to the sharp increase in ARGs in the surface water of the bay.
抗生素的不合理使用导致沿海港湾中抗生素耐药基因（ARGs）的大量繁殖。在陆海相互作用影响下，沿海港湾中 ARGs 的季节性分布鲜有报道。本研究考察了陆海相互作用影响下的石蜊湾中 ARGs 的季节性和空间分布。检测和定量了包括 tetB、tetG、tetX、sul1、sul2、qnrA、qnrB、qnrS、ermF、ermT 和 1 类整合子整合酶基因（intI1）在内的 10 种 ARGs。intI1 和大多数 ARGs 的相对丰度处于 1×10-2 拷贝/16S rRNA 拷贝的数量级。秋季和夏季的总 ARGs 丰度明显高于其他季节。河口、港口和养殖场是该海湾中 ARGs 的重要储存库。沿海水中的营养水平与大多数 ARGs 和 intI1 呈正相关，表明水质是 ARGs 及其传播的重要驱动因素。陆源排放和海水分层被证明是沿海港湾中 ARGs 季节性分布的主要驱动因素。从春季到夏季，陆源排放和海水分层增强，导致湾内表层水中 ARGs 急剧增加。
