分散液液微萃取法快速、廉价测定醋中四甲基吡嗪。
Dispersive liquid-liquid microextraction for rapid and inexpensive determination of tetramethylpyrazine in vinegar.
机构信息
National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
出版信息
Food Chem. 2019 Jul 15;286:141-145. doi: 10.1016/j.foodchem.2019.01.159. Epub 2019 Feb 1.
The concentration of tetramethylpyrazine (TMP) in vinegar is an active indicator of vinegar quality. Dispersive liquid-liquid microextraction (DLLME) was first applied to vinegar as a clean-up pre-treatment for the rapid (5 min) determination of TMP by high-pressure liquid chromatography with ultraviolet detection (HPLC-UV), and may serve as an alternative to solid-phase extraction (SPE) or solid-phase microextraction (SPME). High sensitivity of HPLC for TMP determination was obtained using the DLLME pretreatment, with a limit of detection (LOD) of 0.001 mg L and limit of quantification (LOQ) of 0.005 mg L. The developed method exhibited excellent linearity in the concentration range of 0.050-80.000 mg L, with a correlation coefficient R > 0.999. Furthermore, the percentage recovery of TMP in vinegar using the developed method was within the range 97.97-105.24%. Therefore, DLLME coupled with HPLC-UV is a sensitive and promising method for vinegar clean-up and TMP assay.
四甲基吡嗪（TMP）在醋中的浓度是醋质量的一个活跃指标。分散液液微萃取（DLLME）首先被应用于醋中，作为一种快速（5 分钟）测定高效液相色谱法与紫外检测（HPLC-UV）中 TMP 的净化预处理方法，并且可以替代固相萃取（SPE）或固相微萃取（SPME）。使用 DLLME 预处理，HPLC 对 TMP 的测定具有很高的灵敏度，检测限（LOD）为 0.001mg/L，定量限（LOQ）为 0.005mg/L。所开发的方法在 0.050-80.000mg/L 的浓度范围内表现出极好的线性，相关系数 R>0.999。此外，用所开发的方法测定醋中 TMP 的回收率在 97.97-105.24%范围内。因此，DLLME 与 HPLC-UV 相结合是一种用于醋净化和 TMP 测定的灵敏且有前景的方法。
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