School of Agriculture and Food Science, Zhejiang A & F University, Wusu Street # 666, Lin'an District, Hangzhou 311300, People's Republic of China; College of Food Science and Engineering, Shanxi Agricultural University, Taigu County, Shanxi 030801, People's Republic of China.
School of Agriculture and Food Science, Zhejiang A & F University, Wusu Street # 666, Lin'an District, Hangzhou 311300, People's Republic of China.
Food Chem. 2019 Jul 15;286:329-337. doi: 10.1016/j.foodchem.2019.02.027. Epub 2019 Feb 14.
The effects of exogenous progesterone (PROG) on chilling injury (CI) in postharvest banana fruit were investigated. Concentration screening tests showed that 10 mol/l PROG was most effective in reducing CI in banana fruit stored for 25 d at 5 ± 1 °C, but did not markly increase PROG content of pulps. This PROG treatment significantly reduced the electrolyte leakage, levels of malondialdehyde, O production rate and HO contents in banana compared with control fruit. The PROG treatment caused an early induction of alternative oxidase (AOX) at the transcript and protein level to reduce the generation of O and HO. PROG treatment also enhanced the transcript levels and activities of antioxidant enzymes and maintained higher levels of reduced glutathione and ascorbic acid than the control fruit. These results suggested that PROG attenuating CI in banana fruit may be attributed to the induction of AOX and the improvement of enzyme and non-enzymatic antioxidant defenses.
研究了外源孕酮（PROG）对采后香蕉果实冷害（CI）的影响。浓度筛选试验表明，10mol/L PROG 可最有效地减少在 5±1°C 下贮藏 25d 的香蕉果实的 CI，但不会明显增加果肉中的 PROG 含量。与对照果实相比，该 PROG 处理显著降低了香蕉的电解质渗漏、丙二醛含量、O 产生速率和 HO 含量。PROG 处理还可早期诱导交替氧化酶（AOX）在转录和蛋白水平上的表达，从而减少 O 和 HO 的生成。PROG 处理还增强了抗氧化酶的转录水平和活性，并保持比对照果实更高的还原型谷胱甘肽和抗坏血酸水平。这些结果表明，PROG 减轻香蕉果实的 CI 可能归因于 AOX 的诱导以及酶和非酶抗氧化防御的改善。
