Lin Yuzhao, Lin Hetong, Zeng Lingzhen, Zheng Yi, Chen Yazhen, Fan Zhongqi, Lin Yifen
Institute of Postharvest Technology of Agricultural Products, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
Key Laboratory of Postharvest Biology of Subtropical Special Agricultural Products, Fujian Province University, Fuzhou, Fujian 350002, China.
Food Chem X. 2022 May 25;14:100348. doi: 10.1016/j.fochx.2022.100348. eCollection 2022 Jun 30.
Compared with the -infected longan, the DNP-treated -infected fruit represented a higher pulp breakdown index, a higher O production rate, and a higher MDA content, but the lower activities of APX, SOD and CAT, the lower transcript levels of , , , and , the lower values of AsA, GSH, flavonoid and total phenolics, a lower scavenging ability of DPPH radical, and a lower value of reducing power. Whereas, the ATP-treated -infected samples showed the contrary results. The above findings indicated that the DNP-promoted the pulp breakdown in -infected longan was because DNP weakened the capacity of scavenging ROS, raised the O level, and accelerated the membrane lipids peroxidation. However, the ATP-suppressed the pulp breakdown in -infected longan was because ATP improved the capacity of scavenging ROS, reduced the O level, and reduced the membrane lipids peroxidation.
与感染病菌的龙眼相比，经二硝基酚（DNP）处理的感染病菌果实表现出更高的果肉败坏指数、更高的超氧阴离子（O₂⁻）产生速率和更高的丙二醛（MDA）含量，但抗坏血酸过氧化物酶（APX）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性更低，相关基因的转录水平更低，抗坏血酸（AsA）、谷胱甘肽（GSH）、类黄酮和总酚的含量更低，对1,1-二苯基-2-三硝基苯肼（DPPH）自由基的清除能力更低，以及还原能力更低。然而，经腺嘌呤核苷三磷酸（ATP）处理的感染病菌样品呈现出相反的结果。上述研究结果表明，DNP促进感染病菌的龙眼果肉败坏是因为DNP削弱了活性氧（ROS）清除能力，提高了O₂⁻水平，并加速了膜脂过氧化。然而，ATP抑制感染病菌的龙眼果肉败坏是因为ATP提高了ROS清除能力，降低了O₂⁻水平，并减少了膜脂过氧化。
