Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China; College of Food Science and Technology, Hainan University, Haikou 570228, China.
J Proteomics. 2018 Sep 15;187:1-12. doi: 10.1016/j.jprot.2018.05.011. Epub 2018 May 28.
The mechanism of 24-epibrassinolide (EBR)-induced chilling tolerance in harvested banana fruit was investigated. Results showed that EBR pretreatment remarkably suppressed the development of chilling injury (CI) in harvested banana fruit during 12 days of cold storage at 8 °C, as indicated by lower CI index in treated fruit. Physiological measurements exhibited that EBR treatment reduced the relative electrolyte leakage and malondialdehyde (MDA) content while increased the chlorophyll fluorescence (Fv/Fm), total soluble solids (TSS) and ratio of TSS and titratable acidity. Furthermore, the differentially accumulated proteins of banana fruit in response to EBR and cold treatment were investigated by employing gel-based proteomic in combination with MALDI-TOF-TOF MS and LC-ESI-MS/MS analyses. There were fifty five protein spots to be successfully identified. Notably, most of up-regulated proteins by EBR treatment were related to energy biosynthesis, stress response and cell wall modification. In contrast, proteins involved in protein degradation and energy consumption were down-regulated by EBR treatment. These results suggest that EBR treatment could enhance the defense ability, promote the synthesis and utilization of energy, as well as maintain the protein function via enhancing protein biosynthesis and inhibiting protein degradation, consequently contributing to improvement of cold tolerance in harvested banana fruit.
To extend our understanding of chilling injury (CI) of harvested banana fruit, we reported the effect of 24-epibrassinolide (EBR) on CI of banana fruit when stored at 8 °C. It was the first report on the comprehensive proteomic analysis of banana fruit in response to EBR treatment at low temperature. EBR pretreatment significantly reduced CI in harvested banana fruit. Fifty five protein spots were successfully identified. Notably, the most of up-regulated proteins by EBR treatment were related to energy biosynthesis, stress response and cell wall modification. In contrast, proteins involved in protein degradation and energy consumption were down-regulated. These results suggest that exogenous EBR treatment could enhance the defense ability and maintain high energy status. Meanwhile, EBR treatment maintained protein function via enhancing protein biosynthesis and inhibiting protein degradation. These results may help us to understand the molecular mechanism of the chilling tolerance induced by EBR treatment and broaden the current knowledge of the mechanism of CI of harvested banana fruit.
研究了 24-表油菜素内酯（EBR）诱导采后香蕉果实耐冷性的机制。结果表明，EBR 预处理在 8°C 冷藏 12 天期间显著抑制了采后香蕉果实冷害（CI）的发展，处理过的果实的 CI 指数较低。生理测量显示，EBR 处理降低了相对电解质渗漏和丙二醛（MDA）含量，同时增加了叶绿素荧光（Fv/Fm）、总可溶性固体（TSS）和 TSS 与滴定酸度的比值。此外，通过结合 MALDI-TOF-TOF MS 和 LC-ESI-MS/MS 分析，采用基于凝胶的蛋白质组学研究了香蕉果实对 EBR 和冷处理的差异积累蛋白。成功鉴定了 55 个蛋白斑点。值得注意的是，EBR 处理上调的大多数蛋白与能量生物合成、应激反应和细胞壁修饰有关。相比之下，EBR 处理下调了与蛋白质降解和能量消耗有关的蛋白质。这些结果表明，EBR 处理可以通过增强蛋白质生物合成和抑制蛋白质降解来增强防御能力、促进能量的合成和利用，从而有助于提高采后香蕉果实的耐冷性。
为了进一步了解采后香蕉果实的冷害（CI），我们报道了 24-表油菜素内酯（EBR）对 8°C 贮藏香蕉果实 CI 的影响。这是首次报道 EBR 处理在低温下对香蕉果实的综合蛋白质组学分析。EBR 预处理显著降低了采后香蕉果实的 CI。成功鉴定了 55 个蛋白斑点。值得注意的是，EBR 处理上调的大多数蛋白与能量生物合成、应激反应和细胞壁修饰有关。相比之下，与蛋白质降解和能量消耗有关的蛋白质被下调。这些结果表明，外源 EBR 处理可以增强防御能力并维持高能状态。同时，EBR 处理通过增强蛋白质生物合成和抑制蛋白质降解来维持蛋白质功能。这些结果可能有助于我们理解 EBR 处理诱导的耐冷性的分子机制，并拓宽我们对采后香蕉果实冷害机制的现有认识。
