Morín Jaime G, Venera-Pontón Dagoberto E, Driskell Amy C, Sánchez Juan A, Lasker Howard R, Collin Rachel
Laboratorio de Sistemática Molecular y Filogeografía, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Peru Laboratorio de Sistemática Molecular y Filogeografía, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos Lima Peru.
Smithsonian Tropical Research Institute, Panama City, Panama Smithsonian Tropical Research Institute Panama City Panama.
Biodivers Data J. 2019 Feb 20(7):e30970. doi: 10.3897/BDJ.7.e30970. eCollection 2019.
DNA barcoding is a useful tool for documenting the diversity of metazoans. The most commonly used barcode markers, 16S and COI, are not considered suitable for species identification within some "basal" phyla of metazoans. Nevertheless metabarcoding studies of bulk mixed samples commonly use these markers and may obtain sequences for "basal" phyla. We sequenced mitochondrial DNA fragments of cytochrome oxidase c subunit I (COI), 16S ribosomal RNA (16S), NADH dehydrogenase subunits 2 (16S-ND2), 6 (ND6-ND3) and 4L (ND4L-MSH) for 27 species of Caribbean octocorals to create a reference barcode dataset and to compare the utility of COI and 16S to other markers more typically used for octocorals. The most common genera (, , , , and ) were effectively distinguished by small differences (5 or more substitutions or indels) in COI and 16S sequences. and were effectively distinguished from each other by unique haplotypes, but the small genetic differences make distance approaches ineffective for these taxa. and were indistinguishable from each other but were generally effectively distinguished from other genera, further supporting the idea that these genera have undergone a rapid endemic radiation in the Caribbean.
DNA条形码是记录后生动物多样性的一种有用工具。最常用的条形码标记16S和细胞色素氧化酶亚基I（COI），并不被认为适用于后生动物某些“基部”门类内的物种鉴定。然而，对混合样本的元条形码研究通常使用这些标记，并且可能获得“基部”门类的序列。我们对27种加勒比海八放珊瑚的细胞色素氧化酶c亚基I（COI）、16S核糖体RNA（16S）、烟酰胺腺嘌呤二核苷酸脱氢酶亚基2（16S-ND2）、6（ND6-ND3）和4L（ND4L-MSH）的线粒体DNA片段进行了测序，以创建一个参考条形码数据集，并比较COI和16S与更常用于八放珊瑚的其他标记的效用。最常见的属（、、、、和）通过COI和16S序列中的微小差异（5个或更多替换或插入缺失）得以有效区分。和通过独特的单倍型彼此有效区分，但微小的遗传差异使得距离法对这些分类群无效。和彼此无法区分，但通常能与其他属有效区分，这进一步支持了这些属在加勒比海经历了快速的特有辐射的观点。
