Wang Gang-Tao, Wang Zheng-Feng, Wang Rui-Jiang, Liang Dan, Jiang Guo-Bin
Key Laboratory of Plant Resources Conservation and Sustainable Utilization South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 People's Republic of China.
Guangdong Provincial Key Laboratory of Applied Botany South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 People's Republic of China.
Appl Plant Sci. 2019 Feb 5;7(2):e01217. doi: 10.1002/aps3.1217. eCollection 2019 Feb.
Microsatellite markers were developed to facilitate studies of genetic diversity and structure in , a critically endangered and monotypic conifer species.
Using restriction site-associated DNA sequencing (RAD-Seq), we developed 10 polymorphic and 27 monomorphic microsatellite markers. Polymorphism was characterized using 333 individuals from nine populations. The number of alleles per locus ranged from one to 14 at the population level. The levels of observed and unbiased expected heterozygosities varied from 0.058 to 0.844 and 0.219 to 0.583, respectively. Nine of these 10 polymorphic markers were successfully cross-amplified in , the species most closely related to .
These microsatellite markers can be used to reveal the genetic diversity in existing populations of , enabling its conservation and restoration.
开发微卫星标记以促进对一种极度濒危的单型针叶树种的遗传多样性和结构的研究。
利用限制性位点关联DNA测序（RAD-Seq），我们开发了10个多态性微卫星标记和27个单态性微卫星标记。使用来自9个种群的333个个体对多态性进行了表征。在种群水平上，每个位点的等位基因数从1到14不等。观察到的杂合度和无偏期望杂合度水平分别从0.058到0.844和0.219到0.583不等。这10个多态性标记中的9个在与该树种关系最密切的物种中成功进行了交叉扩增。
这些微卫星标记可用于揭示该树种现有种群的遗传多样性，从而实现其保护和恢复。
